Vincristine Analysis Service

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About vincristine

Vincristine is an alkaloid extracted from the periwinkle plant of the family Oleaceae. Vincristine has the effect of blocking cell division (mitosis), similar to colchicine, but its effect is stronger than colchicine.

Vincristine can fight against cancer, and its efficacy is about 10 times higher than that of vincristine. It is used clinically as one of the anti-cancer drugs and is particularly effective against tumors of hematopoietic organs.

The chemical formula is C46H56N4O10 and the CAS number is 57-22-7.

Creative Proteomics provides a technical platform for the qualitative and quantitative analysis of vincristine.

Molecular structure of vincristine

Platform

LC-MS/MS
GC-MS/MS
NMR

Workflow of targeted metabolomics service of vincristine

Targeted metabolomics is a study that identifies and quantitatively analyzes known metabolites in a sample. Its advantages are high specificity and accuracy. Therefore, this method has been widely used to analyze and compare multiple target metabolites in different physiological states.

Workflow of targeted metabolomics service of vincristine

Sample requirements

Sample demand
Fresh Plant > 1 g / sample, animal tissue 200 mg / sample Cells or bacteria > 10⁷, supernatant > 2 ml Animal body fluid samples > 200 µl Lyophilized samples > 0.5 g
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample. ※ The same condition refers to the same period, basically the same phenotype, and the same part. It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature. Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation). * For varieties with special requirements or rare varieties, please contact our staff for more information.

Delivery standard

Experimental procedure
Parameters of HPLC and MS
Raw data, chromatograms and mass spectra
Metabolites quantification data
Custom analysis report

Project cycle

It takes about 1-3 weeks.

Creative Proteomics has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

Guo H, Jiao Y, Wang X, et al. Twins labeling-liquid chromatography / mass spectrometry-based metabolomics for absolute quantification of tryptophan and its key metabolites. Journal of Chromatography A, 2017, 1504: 83-90.
Kim H, Bu Y, Lee B J, et al. Myristica fragrans seed extract protects against dextran sulfate sodium-induced colitis in mice. Journal of Medicinal Food, 2013, 16(10):953.
Yao Y, Wang G B, Man S L, et al. Toxicological advances of traditional medicine in 2019. Traditional Medical Research (TMR), 2020, 005(002) :P.83-89.

For Research Use Only. Not for use in diagnostic procedures.

Alkaloids

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