Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Theophylline Analysis Service

Theophylline Analysis Service

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About theophylline

Theophylline is a methyl purine drug. It has the effects of cardiotonic, diuretic, coronary artery dilatation, bronchial smooth muscle diastole and central meridian excitation. It is mainly used for the treatment of bronchial asthma, emphysema, bronchitis, cardiogenic dyspnea, etc.

Molecular formula is C7H8N4O2, CAS No. 58-55-9.

Targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, so as to guide your research with reliable and accurate measurement results. Based on the most advanced separation and analysis platform, Creative Proteomics provides targeted metabolomics services. Our experienced technicians continue to improve the detection system and optimize methods to provide the best service to meet customer needs.

Molecular structure of theophyllineMolecular structure of theophylline

Platform

  • LC-MS/MS
  • GC-MS/MS
  • NMR

Workflow of targeted metabolomics service of theophylline

Targeted metabolomics is a study that identifies and quantitatively analyzes known metabolites in a sample. Its advantages are high specificity and accuracy. Therefore, this method has been widely used to analyze and compare multiple target metabolites in different physiological states.

Workflow of targeted metabolomics service of theophylline

Sample requirements

Sample demand
Fresh Plant > 1 g / sample, animal tissue 200 mg / sample
Cells or bacteria > 107, supernatant > 2 ml
Animal body fluid samples > 200 µl
Lyophilized samples > 0.5 g
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature.
Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation).
* For varieties with special requirements or rare varieties, please contact our staff for more information.

Delivery standard

  • Experimental procedure
  • Parameters of HPLC and MS
  • Raw data, chromatograms and mass spectra
  • Metabolites quantification data
  • Custom analysis report

Project cycle

It takes about 1-3 weeks.

Creative Proteomics has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Singh A. Discovery of novel genes and enzymes involved in amaryllidaceae alkaloid biosynthesis using integrated metabolomics and transcriptomics in Narcissus pseudonarcissus King Alfred[D]. Université du Québec à Trois-Rivières, 2018.
  2. Xian Y F, Su Z R, Chen J N, et al. Isorhynchophylline improves learning and memory impairments induced by D-galactose in mice[J]. Neurochemistry International, 2014.
  3. Kim H, Bu Y, Lee B J, et al. Myristica fragrans seed extract protects against dextran sulfate sodium-induced colitis in mice. [J]. Journal of Medicinal Food, 2013, 16(10):953.
For Research Use Only. Not for use in diagnostic procedures.

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