Aconitine is a bis-ester alkaloid, a chemical contained in the commonly used herb aconitine. Aconitine has analgesic effects and is used clinically to relieve cancer pain, especially digestive pain. When applied locally, it paralyzes the surrounding nerve endings, producing local anesthetic and analgesic effects. It also has anti-inflammatory effects. Aconitine is extremely toxic, excites and paralyzes sensory and central nerves, excites the heart and vagus nerve, and directly intoxicates cardiac muscle cells. It also has sweating effects.
Creative Proteomics uses high performance liquid chromatography (HPLC) and liquid mass spectrometry (LC-MS) techniques to enable efficient and accurate qualitative and quantitative analysis of aconitine.
Molecular structure of aconitine
Workflow of targeted metabolomics service of aconitine
Targeted metabolomics is a study that identifies and quantitatively analyzes known metabolites in a sample. Its advantages are high specificity and accuracy. Therefore, this method has been widely used to analyze and compare multiple target metabolites in different physiological states.
|Fresh Plant > 1 g / sample, animal tissue 200 mg / sample|
Cells or bacteria > 107, supernatant > 2 ml
Animal body fluid samples > 200 µl
Lyophilized samples > 0.5 g
|Sample mixed and repeat|
|In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.|
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.
|Storage and transportation|
|Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature.|
Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation).
* For varieties with special requirements or rare varieties, please contact our staff for more information.
- Experimental procedure
- Parameters of HPLC and MS
- Raw data, chromatograms and mass spectra
- Metabolites quantification data
- Custom analysis report
A standard experiment and analysis process takes about 1-4 weeks.
For Research Use Only. Not for use in diagnostic procedures.