Extremolytes Metabolomics Analysis Service

Creative Proteomics offers specialized Extremolytes Metabolomics Analysis to profile stress-adaptive metabolites from extremophilic organisms.

Utilizing high-resolution platforms like Orbitrap HF-X and Agilent 6495C, we deliver:

• Detection Sensitivity: As low as 10 nM
• Mass Accuracy: <2 ppm
• Identification Confidence: ≥95% with standards and MS/MS spectra
• Pathway Coverage: >120 known osmolyte biosynthesis and stress-response pathways
• High Throughput: Up to 96 samples per batch
• Broad Sample Compatibility: Cultures, biomass, extracts, environmental samples

Applications span industrial microbiology, bioprocess optimization, synthetic biology, agriculture, environmental studies, and biomaterials research.

Overview of Extremolytes

Extremolytes are specialized small organic molecules synthesized by extremophiles—organisms thriving in extreme environments such as hydrothermal vents, hypersaline lakes, and polar ice. These molecules, including ectoine, hydroxyectoine, and bacterioruberin, enable survival under stressors like high temperature, salinity, and radiation. Their unique biochemical properties make them invaluable for industrial biotechnology, agriculture, and environmental science. Creative Proteomics's extremolytes metabolomics service leverages advanced omics technologies to profile these compounds, linking their metabolic pathways to functional applications.

Advantages of Extremolytes Metabolomics Analysis Service

• Detection Sensitivity: As low as 10 nM for major extremolytes (e.g., ectoine, betaine)
• Mass Accuracy: <2 ppm with internal calibration
• Identification Confidence: ≥95% with authentic standards and fragmentation spectra
• Pathway Coverage: >120 known pathways involving osmolyte biosynthesis and transport
• Sample Throughput: Up to 96 samples/run with batch processing optimization

Extremolytes Metabolomics Analysis Service Offered by Creative Proteomics

• Targeted Extremolytes Profiling: Accurate quantification of known extremolytes using authentic standards and MRM (Multiple Reaction Monitoring) mode.
• Untargeted Metabolomics of Stress-Induced Cultures: Comprehensive metabolite fingerprinting under various stress conditions to discover novel osmolytes or pathway shifts.
• Microbial Metabolomics: Metabolic characterization of extremophilic microorganisms cultured in defined liquid media.
• Pathway Mapping & Enrichment Analysis: Identification of extremolyte-associated pathways using KEGG and HMDB databases.
• Multi-Omics Integration: Correlation with proteomic data for holistic insights.
• Customized Solutions: Tailored protocols for unique sample types (e.g., microbial mats, extremophile cultures).

List of Detectable Extremolytes and Pathway-Associated Metabolites

Workflow for Extremolytes Metabolomics Analysis Service

Technology Platform for Extremolytes Metabolomics Analysis Service

• Thermo Fisher Q Exactive™ HF-X Orbitrap MS – High-resolution, full-scan and MS/MS fragmentation with <1 ppm accuracy.
• Agilent 1290 Infinity II UHPLC + 6495C Triple Quadrupole LC/MS – Exceptional sensitivity for targeted quantification.
• Sciex Triple Quad™ 6500+ System – Ultra-sensitive detection with dynamic MRM for low-abundance extremolytes.
• Waters Xevo G2-XS QToF – Robust structural elucidation and exact mass measurements.

Sample Requirements for Extremolytes Metabolomics Analysis Service

Applications of Extremolytes Metabolomics Assay Service

Industrial Microbiology

Screening microbial strains for high-osmotic or heat-resistant production traits.

Bioprocess Optimization

Monitoring stress-induced metabolite shifts during fermentation scale-up.

Synthetic Biology

Designing metabolic circuits for stress-responsive compound biosynthesis.

Agricultural Biotechnology

Investigating plant-microbe interactions under salt or drought stress.

Environmental Adaptation Studies

Profiling extremophiles from saline, thermal, or acidic habitats.

Biomaterials & Cosmetics

Evaluating natural extremolytes for use as bio-protectants or stabilizers.

Q: Can you differentiate between isomeric extremolytes like ectoine and hydroxyectoine?

Yes. We utilize high-resolution chromatographic separation (HILIC-UHPLC) and MS/MS fragmentation pattern analysis, which allows precise differentiation between closely related isomers such as ectoine and hydroxyectoine.

Q: What is the minimum number of samples required for statistical analysis?

We recommend at least three biological replicates per condition to enable robust statistical evaluation such as PCA, clustering, and significance testing (e.g., ANOVA).

Q: Can you analyze extremolytes produced under multiple stress conditions simultaneously?

Absolutely. You can submit samples from different environmental stresses (e.g., high salt, oxidative stress, temperature shifts) in parallel. We will analyze and compare metabolic shifts across conditions.

Q: Will I receive raw mass spectrometry data files?

Yes. Upon project completion, we deliver raw LC-MS/MS data (formats like .raw, .mzXML, or .mzML), processed peak tables, and full metadata documentation for transparency and potential reanalysis.

Q: How stable are extremolytes during sample transportation?

Extremolytes are generally stable when frozen at -80°C. However, for highly accurate quantification, we advise minimizing freeze-thaw cycles and ensuring continuous cold chain logistics (dry ice shipping).

Q: Do you provide custom standards if my extremolyte of interest is not common?

Yes. If you request the quantification of uncommon or novel extremolytes, we can source or synthesize custom standards, or alternatively, perform semi-quantitative analysis based on structural analogs.

Q: Can you assist in pathway reconstruction for novel extremolytes?

Yes. We offer bioinformatics support to infer and map potential biosynthetic pathways using KEGG, MetaCyc, and in-house curated extremophile databases, especially useful for novel compound discovery.

Q: How do you ensure quality control (QC) during the analysis?

Each batch includes multiple QC samples:

• Internal standards for normalization
• Pooled QC samples for batch effect monitoring
• Randomized sample order during MS runs to avoid bias

We track retention time shifts, mass accuracy, and signal intensity drifts in real-time.

Q: What if my sample contains high salt or organic solvents?

Please inform us in advance. We can perform additional desalting, liquid-liquid extraction, or solid-phase extraction steps to reduce matrix effects and prevent instrument contamination.

Q: Do you support targeted follow-up studies after untargeted discovery?

Yes. If untargeted metabolomics reveals interesting extremolytes, we can design a targeted MRM method for precise quantification and validation across expanded sample sets.

Q: How sensitive is your platform for low-abundance extremolytes?

Our triple quadrupole LC-MS system achieves detection limits down to picomolar (pM) levels for major extremolytes like ectoine and betaine, ensuring reliable results even in low-concentration samples.

Q: Can I integrate extremolytes data with transcriptomics or proteomics datasets?

Of course. We provide data formats and pathway annotations compatible with multi-omics integration platforms, enabling holistic insights across metabolite, gene, and protein layers.