O-glycosylated Flavonoids Analysis

Overview

O-glycosylated flavonoids are an important group of flavonoids. The common o-glycosylated flavonoids mainly include quercetin 3-O-β-D-glucuronide and quercetin 7-O-β-D-glucopyranoside. Quercetin 3-O-β-D-glucuronide can be derived from dried whole grasses of hedyotis diffusa willd, flowers of gossypiumherbaceum L, or the leaf of apocynum lanci-folium rus, which can also be prepared by chemical synthesis. Quercetin 3-O-β-D-glucuronide is soluble in DMSO, alkaline water, insoluble in fat-soluble organic solvents. It is a yellow acicular crystal in water, and soluble in alkaline solution shows deep yellow. Quercetin 7-O-β-D-glucopyranoside can be derived from sophora flavescens ait, generally soluble in DMSO, methanol and other solvents, but insoluble in petroleum ether. Because o-glycosylated flavonoids have a wide range of uses, it is very important to establish the method for the analysis of o-glycosylated flavonoids in plant metabolites. High performance liquid chromatography is a sensitive analytical technique that enables detection, identification and quantification of a variety of o-glycosylated flavonoids by characterizing their chemical structure, even at low abundance. Improved extraction and purification steps can also increase the sensitivity of the analytical method.

Applications of O-glycosylated Flavonoids Analysis

• Qualitative analysis of the main types of o-glycosylated flavonoids
• Quantitative analysis of o-glycosylated flavonoids content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our O-glycosylated Flavonoids Analysis Service

Excellent o-glycosylated flavonoids qualitative and quantitative capabilities. Creative Proteomics has rich experience in o-glycosylated flavonoids sample handling and can analyze o-glycosylated flavonoids present in a variety of plant species at cell/tissue level.

Advanced analytical technology platform. We have our own research and testing team, equipped with standard advanced analysis equipment and efficient and precise testing instruments, which is a strong support for us to set up a strict and scientific management system.

Individualized experimental scheme. We can provide one-to-one customized services, assign professional project leaders to analyze the whole project, directly and quickly report the progress to customers, and flexibly adjust the project plan according to customers&#39 opinions.

Service Workflow

Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of various types of o-glycosylated flavonoids, including quercetin 3-O-β-D-glucuronide, quercetin 7-O-β-D-glucopyranoside, etc.

Detector: UV detector

Linear range: R > 0.999

Elution time: dozens of minutes, slightly different according to different ingredients

List of Detectable O-glycosylated Flavonoids at Creative Proteomics

Sample Requirements

1. Fresh tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Deliverables

• Experimental procedure
• Parameters of HPLC
• Purity analysis report
• HPLC raw data files and HPLC data quality checks
• Metabolites quantification data
• Custom analysis report

HPLC-based profiling of o-glycosylated flavonoids enables quantitative analyses of o-glycosylated flavonoids in a faster, convenient, and sensitive manner. With decades of experience in HPLC services, Creative Proteomics has a proven track record supporting diverse o-glycosylated flavonoids detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.