O-glycosylated flavonoids are an important group of flavonoids. The common o-glycosylated flavonoids mainly include quercetin 3-O-β-D-glucuronide and quercetin 7-O-β-D-glucopyranoside. Quercetin 3-O-β-D-glucuronide can be derived from dried whole grasses of hedyotis diffusa willd, flowers of gossypiumherbaceum L, or the leaf of apocynum lanci-folium rus, which can also be prepared by chemical synthesis. Quercetin 3-O-β-D-glucuronide is soluble in DMSO, alkaline water, insoluble in fat-soluble organic solvents. It is a yellow acicular crystal in water, and soluble in alkaline solution shows deep yellow. Quercetin 7-O-β-D-glucopyranoside can be derived from sophora flavescens ait, generally soluble in DMSO, methanol and other solvents, but insoluble in petroleum ether. Because o-glycosylated flavonoids have a wide range of uses, it is very important to establish the method for the analysis of o-glycosylated flavonoids in plant metabolites. High performance liquid chromatography is a sensitive analytical technique that enables detection, identification and quantification of a variety of o-glycosylated flavonoids by characterizing their chemical structure, even at low abundance. Improved extraction and purification steps can also increase the sensitivity of the analytical method.
Applications of O-glycosylated Flavonoids Analysis
- Qualitative analysis of the main types of o-glycosylated flavonoids
- Quantitative analysis of o-glycosylated flavonoids content
- Ensure safety and quality
- Provide basis for improvement
Advantages of Our O-glycosylated Flavonoids Analysis Service
Excellent o-glycosylated flavonoids qualitative and quantitative capabilities. Creative Proteomics has rich experience in o-glycosylated flavonoids sample handling and can analyze o-glycosylated flavonoids present in a variety of plant species at cell/tissue level.
Advanced analytical technology platform. We have our own research and testing team, equipped with standard advanced analysis equipment and efficient and precise testing instruments, which is a strong support for us to set up a strict and scientific management system.
Individualized experimental scheme. We can provide one-to-one customized services, assign professional project leaders to analyze the whole project, directly and quickly report the progress to customers, and flexibly adjust the project plan according to customers' opinions.
Since different tissues have different matrix properties, Creative Proteomics has developed multiple novel sample preparation techniques. Our services allow the profiling of various types of o-glycosylated flavonoids, including quercetin 3-O-β-D-glucuronide, quercetin 7-O-β-D-glucopyranoside, etc.
Detector: UV detector
Linear range: R > 0.999
Elution time: dozens of minutes, slightly different according to different ingredients
List of Detectable O-glycosylated Flavonoids at Creative Proteomics
1. Fresh tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.
2. Product samples.
At least 3 biological replicates.
- Experimental procedure
- Parameters of HPLC
- Purity analysis report
- HPLC raw data files and HPLC data quality checks
- Metabolites quantification data
- Custom analysis report
HPLC-based profiling of o-glycosylated flavonoids enables quantitative analyses of o-glycosylated flavonoids in a faster, convenient, and sensitive manner. With decades of experience in HPLC services, Creative Proteomics has a proven track record supporting diverse o-glycosylated flavonoids detection and quantification. We can meet your specific project requirements, from sampling to bioinformatics.
For Research Use Only. Not for use in diagnostic procedures.