Quercetin 3-O-β-D-Glucuronide Analysis Service

Overview

Quercetin 3-O-β-D-glucuronide can be derived from dried whole grasses of hedyotis diffusa willd, flowers of gossypiumherbaceum L, or the leaf of apocynum lanci-folium rus, which can also be prepared by chemical synthesis. The plant containing quercetn 3-O-β-D-glucuronide can be used as a commonly used clinical medicine, often with the effect of clearing away heat and detoxification, promoting blood circulation and removing blood stasis, lifting dampness and drenching, and can be used for the treatment of cancer, hepatitis, pneumonia and other diseases. Quercetin 3-O-β-D-glucuronide is soluble in DMSO, alkaline water, insoluble in fat-soluble organic solvents. It is a yellow acicular crystal in water, and soluble in alkaline solution shows deep yellow. Because quercetin 3-O-β-D-glucuronide has a wide range of uses, it is very important to establish the method for the analysis of quercetin 3-O-β-D-glucuronide in plant metabolites. Creative Proteomics provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse quercetin 3-O-β-D-glucuronide. High performance liquid chromatography (HPLC) can solve some of the problems associated with quercetin 3-O-β-D-glucuronide analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.

Applications of Quercetin 3-O-β-D-Glucuronide Analysis

• Qualitative analysis of the main types of quercetin 3-O-β-D-glucuronide
• Quantitative analysis of quercetin 3-O-β-D-glucuronide content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our Quercetin 3-O-β-D-glucuronide Analysis Service

Excellent quercetin 3-O-β-D-glucuronide qualitative and quantitative capabilities. We have automatic analysis system, which can efficiently and accurately complete qualitative and quantitative analysis, ensuring that the results are scientific and reliable.

Individualized experimental scheme. We can provide one-to-one customized services, assign professional project leaders to analyze the whole project, directly and quickly report the progress to customers, and flexibly adjust the project plan according to customers' opinions.

Full cycle quality control system.

Professional data analysis skills.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is up to 99%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

Figure 1. Quercetin 3-O-β-D-glucuronide analysis service workflow.

Mobile phase: acetonitrile - glacial acetic acid

Detector: UV detector

Linear range: R > 0.999

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of quercetin 3-O-β-D-glucuronide
• Differential metabolites screening
• Optimal analyses

Sample Requirements

1. Fresh tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC
• Purity analysis report
• HPLC raw data files and HPLC data quality checks
• Metabolites quantification data
• Custom analysis report

HPLC based profiling of quercetin 3-O-β-D-glucuronide enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC services, Creative Proteomics has a proven track record supporting diverse quercetin 3-O-β-D-glucuronide detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.