Quercetin-7-O-β-D-Glucopyranoside Analysis Service

Overview

Quercetin-7-O-β-D-glucopyranoside can be derived from sophora flavescens ait, generally soluble in DMSO, methanol and other solvents, but insoluble in petroleum ether. Because quercetin-7-O-β-D-glucopyranoside has a wide range of uses, it is very important to establish the method for the analysis of quercetin-7-O-β-D-glucopyranoside in plant metabolites. Creative Proteomics provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse quercetin-7-O-β-D-glucopyranoside. High performance liquid chromatography (HPLC) can solve some of the problems associated with quercetin-7-O-β-D-glucopyranoside analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.

Applications of Quercetin-7-O-β-D-glucopyranoside Analysis

• Qualitative analysis of the main types of quercetin-7-O-β-D-glucuronide
• Quantitative analysis of quercetin-7-O-β-D-glucopyranoside content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our quercetin-7-O-β-D-glucopyranoside Analysis Service

Advanced analytical technology platform. We have our own research and testing team, equipped with standard advanced analysis equipment and efficient and precise testing instruments, which is a strong support for us to set up a strict and scientific management system.

Professional testing and analysis team. We have an immeasurable talent advantage, and have established a good cooperative relationship with various scientific research institutions, constantly committed to technological innovation, product innovation and management innovation, so as to ensure that our services have a strong competitive advantage in the same industry.

Excellent quercetin-7-O-β-D-glucopyranoside qualitative and quantitative capabilities. We can efficiently and accurately complete qualitative and quantitative analysis, ensuring that the results are scientific and reliable.

Individualized experimental scheme. We can provide one-to-one customized services, assign professional project leaders to analyze the whole project, directly and quickly report the progress to customers, and flexibly adjust the project plan according to customers' opinions.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is more than 90%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

Figure 1. Quercetin-7-O-β-D-glucopyranoside analysis service workflow.

Mobile phase: Methanol-acetic acid aqueous solution

Detector: UV detector

Linear range: R > 0.999

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of quercetin-7-O-β-D-glucopyranoside
• Differential metabolites screening
• Optimal analyses

Sample Requirements

1. Fresh tissues:  >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC
• Purity analysis report
• HPLC raw data files and HPLC data quality checks
• Metabolites quantification data
• Custom analysis report

HPLC based profiling of quercetin-7-O-β-D-glucopyranoside enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC services, Creative Proteomics has a proven track record supporting diverse quercetin-7-O-β-D-glucopyranoside detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.