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Creative Proteomics Metabolomics

Ephedrine Analysis Service

Ephedrine Analysis Service

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Ephedrine is a substance extracted from the plant Ephedra, which has pharmacological effects, such as raising blood pressure and increasing cardiac output. Creative Proteomics has a high-resolution mass spectrometry detection platform to provide you with targeted metabolomics services for the qualitative and quantitative analysis of ephedrine.

About Ephedrine

Ephedrine is an aromatic secoamine alkaloid, which is present in a variety of ephedra. Its molecular structure is similar to that of phenylpropanolamine, methamphetamine and epinephrine. Ephedrine exhibits optical isomerism, with two chiral centers, giving rise to four stereoisomers. By convention, the pair of enantiomers with stereochemistry (1R,2S) and (1S,2R) is referred to as ephedrine, while the pair of enantiomers with stereochemistry (1R,2R) and (1S,2S) is referred to as pseudoephedrine.

Ephedrine is widely used in pharmaceutical preparations because of its sweating, asthma, diuretic, anti-inflammatory and other effects.

Molecular structure of ephedrineMolecular structure of ephedrine

Ephedrine can make the coronary arteries, brain, muscle vasodilatation, so that myocardial contraction, increase cardiac output, often causing systolic and diastolic blood pressure rise, pulse pressure increases, but also has a lowering effect of blood viscosity. Ephedrine has an excitatory effect on the respiratory center and an expanding effect on bronchial smooth muscle, but the effect is weak, slow and long-lasting, and has a positive effect on the treatment of asthma and other respiratory diseases. Ephedrine can excite the cerebral cortex and subcortical centers, making people feel mentally excited, even insomnia, etc. Ephedrine can increase the secretion of sweat glands by blocking the reabsorption of sodium from the sweat gland ducts, which has an antipyretic effect on patients with fever. Studies have also shown that ephedrine has antibacterial and antiviral effects on Candida albicans, S. typhi, E. coli, S. dysenteriae, P. aeruginosa, S. diphtheriae, Haemophilus influenzae, B. anthracis, B. streptococci, A. streptococci, S. aureus, influenza A virus PR, Asian influenza A virus, etc.

Creative Proteomics provides qualitative and absolute quantitative analysis of ephedrine through high performance liquid chromatography (HPLC) and high-performance liquid chromatography-mass spectrometry (LC-MS) detection platforms.

The four stereoisomers of ephedrineThe four stereoisomers of ephedrine

Targeted Metabolomics Workflow of Ephedrine

Targeted metabolome studies can establish baseline metabolite levels in tissues or organisms for comparison among experiments under different conditions.

Design experiments and sample processing

Standard preparation and mass spectrometry detection, and make a standard curve

Extraction of metabolites in samples

Method verification and data quality control

Raw data preprocessing and absolute quantification of metabolites based on standard curve

Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)

Molecular structure of chlorophyll

Sample Requirements

  • Cell ≥ 200 μL/sample. Centrifuge after sampling, remove the medium, immediately put it in liquid nitrogen, and store it at -80°.
  • Tissue ≥ 200 mg/sample. Please put it in liquid nitrogen immediately after separation and store it at -80°.
  • Plasma ≥ 200 μL/sample.
  • Blood ≥ 200 μL/sample.

Dry ice transportation (dry ice volatilizes 3-4 kg/day, please use sufficient dry ice for transportation).

The experimental samples will not be returned, please back them up by yourself.

Service Process

  • Consultation for details by email or phone
  • Signing a contract
  • Fill in the information sheet
  • Sample submission
  • Detection and analysis
  • Output experiment report
  • Delivery and after-sales Q&A

Delivery Standard

  • Complete lab report (including information on methods, instrument parameters, reagents, and consumables)
  • Raw data
  • Mass spectrum
  • Metabolic molecular identification results

Turnaround time: About 1-4 weeks.

Creative Proteomics has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of targeted metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Michaela B, Simone W, et al. Targeted metabolomics identifies reliable and stable metabolites in human serum and plasma samples[J]. Plos One, 2014, 9(2):e89728.
  2. Lee K M, Jeon J Y, et al. Application of metabolomics to quality control of natural product derived medicines[J]. Biomolecules & Therapeutics, 2017, 25(6):559-568.
  3. Krizevski R, Bar E, et al. Composition and stereochemistry of ephedrine alkaloids accumulation in Ephedra sinica Stapf[J]. Phytochemistry, 2010, 71(8-9):895-903.
  4. Lv M, Chen J, et al. Metabolomics based on liquid chromatography with mass spectrometry reveals the chemical difference in the stems and roots derived from Ephedra sinica[J]. Journal of Separation Science, 2015, 38(19):3331-3336.
For Research Use Only. Not for use in diagnostic procedures.

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