Rutin Analysis Service

Overview

Rutin is a kind of vitamin drug, which is a natural flavonoid glycoside. It can be derived from such plants as rue leaf, tobacco leaf, jujube, apricot, orange peel, tomato, buckwheat flower and so on. Rutin is a light yellow needle-like crystal, soluble in pyridine, formyl and lye, slightly soluble in ethanol, acetone and ethyl acetate, and almost insoluble in chloroform, ether, benzene, carbon disulfide and petroleum ether. Rutin has the effect of reducing capillary permeability and brittleness, and can maintain and restore the normal elasticity of capillary. Clinically, rutin can be used to prevent and cure hypertension, cerebral haemorrhage, diabetic retinal hemorrhage and hemorrhagic purpura. Rutin is also used as a food antioxidant, a pigment and nutrient enhancer. In addition, it can be used as sunscreen whitening cosmetic. Because rutin has a wide range of uses, it is very important to establish the method for the analysis of rutin in plant metabolites. Creative Proteomics provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse rutin. High performance liquid chromatography-mass spectrometry (HPLC-MS/MS) can solve some of the problems associated with rutin analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.

Applications of Rutin Analysis

• Qualitative analysis of the main types of rutin
• Quantitative analysis of rutin content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our rutin Analysis Service

Rich experience in sample handling.

Perfect experimental platform.

High cost performance.

Strong analytical skills.

Impeccable plant metabolomics services.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is up to 99%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

 Figure 1. Rutin analysis service workflow.

Mass spectrometric conditions: electrospray ionization (ESI), multi-reaction monitoring (MRM) mode for positive ion scanning

Mobile phase: acetonitrile-formic acid

Linear range: R2 > 0.999

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantification of rutin
• Differential metabolites screening
• Optimal analyses

Sample Requirements

1. Fresh tissues: >1 g. If possible, samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC-MS/MS
• Purity analysis report
• HPLC-MS/MS raw data files and HPLC-MS/MS data quality checks
• Metabolites quantification data
• Custom analysis report

HPLC-MS/MS based profiling of rutin enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC-MS/MS services, Creative Proteomics has a proven track record supporting diverse rutin detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.