About Erythrose-4-phosphate
Erythrose 4-phosphate is a phosphate of the monosaccharide erythrose. It is an intermediate of the pentose phosphate pathway and Calvin cycle.
In addition, it is also a precursor for the biosynthesis of aromatic amino acids tyrosine, phenylalanine and tryptophan. It is used in the first step of the shikimate pathway. At this stage, phosphoenolpyruvate and 4-phosphate erythroside react in a reaction catalyzed by DAHP synthase to form 3-deoxy-D-arabinoheptulonic acid-7-phosphate (DAHP).
Its chemical formula is C4H9O7P, and CAS number is 585-18-2.
MS-based technical means can realize the qualitative and quantitative analysis of erythrose-4-phosphate.
Molecular structure of erythrose-4-phosphate
Technical Route of Targeted Metabolomics of Erythrose-4-phosphate
Sample Requirements
| Sample type |
Plants, yeasts, microorganisms, etc.
Serum, plasma, urine, bile, bile acid, etc.
Cells, liver, brain tissue and other animal tissues and feces, etc.
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information. |
| Sample demand |
Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 107, supernatant > 2 ml
Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
(Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
Animal tissue / feces related samples > 200 mg / sample
For other sample types, please consult technical support or sales. |
| Sample mixed and repeat |
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates. |
| Storage and transportation |
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80°C.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation). |
Feedback to Customers
Creative Proteomics will provide you with detailed technical reports, including
- Experimental steps
- Related mass spectrometry parameters
- Part of the mass spectrum picture
- Raw data
- Metabolic molecular identification results
Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.
References
- Kim M J, Lee M Y, Shon J C, et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder. Food Chemistry, 2019, 300:125169.
- Pawlak M, Klupczynska A, Kokot Z J, et al. Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids. Toxins, 2019, 12(1):14.
- Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.
For Research Use Only. Not for use in diagnostic procedures.
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