3-phosphoglycerate Analysis Service

What is 3-Phosphoglycerate?

3-Phosphoglycerate (3-PG) is a critical intermediate in the metabolic pathway that converts glucose into energy in the form of ATP. It is also involved in the biosynthesis of various essential molecules like amino acids, lipids, and nucleotides. The measurement of 3-PG levels can provide valuable insights into cellular metabolism, energy status, and disease progression.

Cancer cells have a unique metabolic profile characterized by increased glucose uptake and utilization through glycolysis, even in the presence of oxygen, a phenomenon known as the Warburg effect. 3-PG is an important intermediate in this process and its levels are typically elevated in cancer cells. Therefore, 3-PG analysis can provide valuable information about tumor metabolism and potential drug targets.

Athletes require a constant supply of energy during intense training. 3-PG levels can reflect the energy status of muscle cells. 3-PG analysis can help identify metabolic disorders that may affect an athlete's performance.

3-PG analysis can also be used to study plant metabolism and understand how plants use carbon dioxide for growth and development. It can also provide insight into how plants respond to environmental stresses such as drought or heat.

Liquid chromatography-mass spectrometry (LC-MS) technology is a highly sensitive and high-resolution technique that can detect low levels of molecules in complex biological samples. This technique separates and ionizes the sample, and then detects and identifies it in the mass spectrometer. Because 3-PG concentrations are typically very low, a highly sensitive technique is required for detection.

Creative Proteomics has a team of experienced scientists and we use advanced technology and analytical methods to provide accurate and reliable results for 3-PG analysis. Their services include not only 3-PG analysis, but also analysis of other metabolites such as glucose, lactate and pyruvate. In addition, we offer customized services to meet your specific needs.

Technical Route of Targeted Metabolomics of 3-phosphoglycerate

Feedback to Customers

Creative Proteomics will provide you with detailed technical reports, including

• Experimental steps
• Related mass spectrometry parameters
• Part of the mass spectrum picture
• Raw data
• Metabolic molecular identification results

Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

1. Riera-Borrull M, Rodríguez-Gallego E, Hernández-Aguilera A, et al. Exploring the process of energy generation in pathophysiology by targeted metabolomics: performance of a simple and quantitative method. Journal of the American Society for Mass Spectrometry, 2015, 27(1): 168-177.
2. Kim M J, Lee M Y, Shon J C, et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder. Food Chemistry, 2019, 300:125169.
3. Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.