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Nicotinamide Adenine Dinucleotide Phosphate Analysis Service

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What is Nicotinamide Adenine Dinucleotide Phosphate?

Nicotinamide adenine dinucleotide phosphate (NADP) is a coenzyme found in living cells that plays an essential role in energy metabolism. It is an oxidizing agent that can accept electrons during the process of cellular respiration, thereby participating in the production of ATP, the main energy currency of the cell. NADP is also involved in other biochemical processes, such as the biosynthesis of fatty acids and cholesterol, and the regulation of gene expression. Therefore, accurate measurement of NADP levels is crucial for understanding the cellular function and disease mechanisms.

LC-MS is a powerful analytical technique used for the quantification of small molecules, such as NADP, in biological samples. It combines liquid chromatography (LC) with mass spectrometry (MS) to separate and detect analytes based on their physicochemical properties and mass-to-charge ratio, respectively. LC-MS provides high sensitivity, specificity, and accuracy, making it a preferred method for NADP analysis in complex biological matrices.

Creative Proteomics is a leading provider of LC-MS-based services for NADP analysis. Our state-of-the-art facilities and experienced scientists can handle projects of any size and complexity, from method development to sample preparation and data interpretation.

Application of NADP Analysis

  • Measurement of NADP levels in various metabolic pathways, such as glycolysis, TCA cycle and pentose phosphate pathway. changes in NADP levels can indicate alterations in these pathways due to genetic, environmental or pathological factors, helping to elucidate the underlying mechanisms of metabolic disorders such as diabetes, obesity and cardiovascular disease. In addition, LC-MS can be used to quantify levels of NADP+ and NADPH. dysregulation of the NADP/NADPH ratio has been associated with various pathologies such as inflammation, aging and neurodegeneration.
  • Cancer cells have high energy and biosynthetic demands and NADP metabolism is often altered. Measuring NADP levels in cancer cells and tissues will help to study the effects of anticancer drugs on NADP metabolism.
  • The metabolism of NADP is associated with the aging process. Measuring NADP levels in aging tissues investigates the effects of NADP precursors, such as nicotinamide riboside and nicotinamide mononucleotide, on aging-related phenotypes.
  • Measurement of NADP levels in brain tissue to study the effects of NADP precursors and modulators on cognitive and motor function.
  • Measurement of NADP levels in microbial cultures to study the effects of NADP regulators and inhibitors on microbial growth and metabolism
  • Measurement of NADP levels in plant tissues to study the effects of NADP regulators and inhibitors on plant growth and stress resistance.

Molecular structure of nicotinamide adenine dinucleotide phosphateMolecular structure of nicotinamide adenine dinucleotide phosphate

Technical Route of Targeted Metabolomics of Nicotinamide Adenine Dinucleotide Phosphate

Technical Route of Targeted Metabolomics of Nicotinamide Adenine Dinucleotide Phosphate

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Creative Proteomics will provide you with detailed technical reports, including

  • Experimental steps
  • Related mass spectrometry parameters
  • Part of the mass spectrum picture
  • Raw data
  • Metabolic molecular identification results

Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.


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  2. Kim M J, Lee M Y, Shon J C, et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder. Food Chemistry, 2019, 300:125169.
  3. Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.
For Research Use Only. Not for use in diagnostic procedures.


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