NAD⁺ Precursor (NMNs) Analysis

Nicotinamide mononucleotide (NMN) is an intermediate in the remedial synthesis pathway of nicotinamide adenine dinucleotide (NAD+) in mammals. By regulating the level of NMN in organisms, it has better therapeutic and reparative effects on cardiovascular and cerebrovascular diseases, neurodegenerative diseases and degenerative diseases of aging. In addition, NMN can also play a role in protecting and repairing pancreatic islet function, increasing insulin secretion and preventing metabolic diseases such as diabetes and obesity by participating in and regulating the endocrine of the organism. In view of the biological activity of NMN, the development of drugs with NMN as the active ingredient has become a medical hotspot. NMN is widely found in natural foods, and has been found in vegetables, fungi, meat and shrimp. Functional foods with NMN as the active ingredient are also in the exploration and development stage.

Targeted and specific detection and analysis for a particular sample requires high sensitivity and quantitative accuracy of the assay. Creative Proteomics relies on accurate quantitative, stable and easy-to-use LC-QQQ triple quadrupole liquid mass spectrometry (LC / MS), GC-QQQ triple quadrupole gas mass spectrometry (GC / MS) and unique ion chromatography mass spectrometry (ICMS) for accurate characterization and quantification of nicotinamide mononucleotides. Different platforms can be selected for different targets.

Molecular structure of nicotinamide mononucleotide

Technical Route of Nicotinamide Mononucleotide Analysis

Sample Requirements

• Plant tissue ＞ 200 mg fresh weight, freeze-dried sample ＞ 0.5 g
• Animal tissue / feces related samples ＞ 200 mg / sample
• Cells or bacteria ＞ 10⁷, supernatant ＞ 2 ml
• Amniotic fluid and other homogeneous liquid samples ＞ 200 µl.

In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample, and prepare more than 6 biological replicates.

Quick-frozen preservation with liquid nitrogen can minimize the leakage time of samples at room temperature, and store it at -80°C.

Feedback to customers

Creative Proteomics will provide you with detailed technical reports, including

• Experimental steps and related mass spectrometry parameters
• Metabolic molecular identification results
• Part of the mass spectrum pictures

Creative Proteomics offers several approaches to provide nicotinamide mononucleotide quantitative analysis service. We will deliver precise and detailed data and analysis report. If you want to detect other substances, you can tell us through the inquiry form, and our technicians will communicate with you.