Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Fructose-6-phosphate Analysis Service

Fructose-6-phosphate Analysis Service

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Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

About Fructose-6-phosphate

Fructose 6-phosphate is one of the common molecules in organisms and one of the products produced in the process of glycolysis. It belongs to ketose. In glycolysis, fructose 6-phosphate is formed by glucose 6-phosphate under the catalysis of phosphate glucose isomerase. Afterwards, it will be catalyzed by phosphofructokinase and consume an ATP to generate fructose 1,6-bisphosphate, which is the second phosphorylation in glycolysis.

Its chemical formula is C6H13O9P, and CAS number is 643-13-0.

MS-based technical means can realize the qualitative and quantitative analysis of fructose-6-phosphate.

Molecular structure of fructose-6-phosphateMolecular structure of fructose-6-phosphate

Technical Route of Targeted Metabolomics of Fructose-6-phosphate

Technical Route of Targeted Metabolomics of Fructose-6-phosphate

Sample Requirements

Sample type
Plants, yeasts, microorganisms, etc.
Serum, plasma, urine, bile, bile acid, etc.
Cells, liver, brain tissue and other animal tissues and feces, etc.
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.
Sample demand
Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 107, supernatant > 2 ml
Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
(Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
Animal tissue / feces related samples > 200 mg / sample
For other sample types, please consult technical support or sales.
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

  • Experimental steps
  • Related mass spectrometry parameters
  • Part of the mass spectrum picture
  • Raw data
  • Metabolic molecular identification results

Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Kim M J, Lee M Y, Shon J C, et al. Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder. Food Chemistry, 2019, 300:125169.
  2. Pawlak M, Klupczynska A, Kokot Z J, et al. Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids. Toxins, 2019, 12(1):14.
  3. Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.
For Research Use Only. Not for use in diagnostic procedures.
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