Propanoate Metabolism Service

Overview

Propanoate metabolism depicts the metabolism of propionic acid. Propionic acid basically stems from the production of the acid by either gut or skin microflora. Propanoate is converted into propanol adenylate via propionyl-CoA synthetase and acetyl-CoA synthetase in mitochondrial. The propionyl adenylate then is converted into propionyl coenzyme A (propionyl-CoA), which is carboxylated into D-methylmalonyl-CoA via propionyl-CoA carboxylase. The resulting compound is isomerized into L-methylmalonyl-CoA via methylmalonyl-CoA epimerase. L-methylmalonyl-CoA can enter into citric acid cycle by converting into succinyl-CoA via methylmalonyl CoA mutase. As one of important carbohydrate metabolism related metabolic pathways, propanoate metabolism plays important roles in physiological conditions. Its aberrant is closely associated with various diseases like primary propionic acidemia and malonyl-CoA decarboxylase deficiency. In Creative Proteomics, we provide reliable, rapid and cost-effective propanoate metabolism analysis service to speed up diseases diagnosis and gain new insights in disease mechanism or treatment.

The Main Applications of Propanoate Metabolism Service

Identifying novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of drugs

Gaining new mechanism of metabolic diseases

Advantages of Our Propanoate Metabolism Service

Ample experience in a variety of animal sample preparation

Advanced core facilities

Professional experiment design and data analysis

Low cost

Short experimental time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides propanoate metabolism service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four major steps: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

 Figure 1. The overall workflow of propanoate metabolism service.

The Potential Detectable Propanoate Metabolism Related Metabolites at Creative Proteomics

Propanol adenylate	Propionyl-CoA	D-methylmalonyl-CoA
L-methylmalonyl-CoA	Succinyl-CoA	Methylmalonate

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the below requirements for different kinds of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of propanoate metabolism, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored propanoate metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. William R Wikoff, Jon A Gangoiti, Bruce A Barshop, et al. Metabolomics identifies perturbations in human disorders of propionate metabolism. Clin Chem. 2007,53:2169-2176.
2. D R FitzPatrick, A Hill, J L Tolmie, et al.  Infantile cerebellar-retinal degeneration associated with a mutation in mitochondrial aconitase, ACO2. Am J Hum Genet. 1999,65:318-326.