Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.
About Allylglucosinolate (Sinigrin)
The chemical name of sinigrin is allyl glucosinolate or 2-propenyl glucosinolate.
Sinigrin is a major glucosinolate, found in some Brassica plants, such as Brassica nigra, Brussels sprouts and broccoli seeds.
Allylglucosinolate (sinigrin) is the precursor of allyl isothiocyanate. Whenever plant tissues containing myrosin are crushed or otherwise damaged, myrosinase degrades myrosin into mustard oil (allyl isothiocyanate), which is the reason for the spicy taste of mustard and horseradish.
The metabolic activation of Sinigrin leads to the formation of isothiocyanates, which have a variety of therapeutic activities, such as anti-cancer, anti-inflammatory, antibacterial, anti-fungal, anti-oxidation, wound healing, and biological fumigation.
It is also used as a nutritional supplement, because it has preventive and medicinal value for various diseases.
Its molecular formula is C10H16KNO9S2, and its CAS number is 3952-98-5.
MS-based technical means can realize the qualitative and quantitative analysis of allylglucosinolate (sinigrin).
Structure of sinigrin
Sinigrin sources and its therapeutic activity (picture from Mazumder, Anisha, et al., 2016)
Waters ACQUITY UPLC, MS-SCIEX QTRAP 4500/5500/6500 and Waters Xevo TQ-S
Agilent 7890B-5977A, Thermo TRACE 1300-TSQ 9000
- High sensitivity
The sensitivity can reach pg level, and it can accurately quantify low-abundance metabolites.
- Qualitative and quantitative standards
Each metabolite is qualitatively and quantitatively compared with standard products to ensure the accuracy of the results.
- Accurate quantification of allylglucosinolate (sinigrin)
- Validate hypotheses proposed by non-targeted metabolomics or other omics
- Research metabolic models for allylglucosinolate (sinigrin)
Technical Route of Targeted Metabolomics of Allylglucosinolate (Sinigrin)
Sample Preparation Suggestions
|Sample type||Suggested sample quantity (per case)||Number of repetitions (recommended)|
|Plant tissue||≥1g||≥ 6|
|Animals and clinical organizations||≥200 mg||≥ 10|
|Serum, plasma||≥200 mg||≥ 6|
|Urine||≥200 μl||≥ 6|
|Stool, intestinal contents||≥500 μl||≥ 6|
|Microorganism||≥107 or ≥100 mg||≥ 6|
|Culture broth, fermentation broth||≥1 ml||≥ 6|
Feedback to Customers
Creative Proteomics will provide you with detailed technical reports, including
- Experimental steps
- Related mass spectrometry parameters
- Part of the mass spectrum picture
- Raw data
- Quantitative results of targeted metabolites
- A standard experimental procedure takes about 1 ~ 4 weeks.
- Mazumder, Anisha, et al. Sinigrin and Its Therapeutic Benefits[J]. Molecules, 2016.
For Research Use Only. Not for use in diagnostic procedures.