Warfarin Analysis Service

Overview

Warfarin is a coumarin-based oral anticoagulant with a chemical structure similar to that of vitamin K, which competes against the effects of vitamin K. By inhibiting carboxylase synthesis, warfarin interferes with the conversion of vitamin K into active coagulation factors II, VII, IX and X in hepatocytes, thus exerting an anticoagulant effect. Warfarin is used clinically for the prevention and treatment of thrombotic disorders, including atrial fibrillation, post-operative heart valve replacement, pulmonary embolism, and deep vein thrombosis. In addition, warfarin can be made into powder, microcapsules, or its sodium salt dissolved in water to poison rats and other rodents. Creative Proteomics provides qualitative and quantitative warfarin analysis services to facilitate a wide range of basic research that will lead to safer and more effective clinical applications of warfarin.

What We Can Offer

• Content analysis of warfarin components
• Qualitative analysis of the main types of warfarin
• Identification and quantification of warfarin chiral compounds and isomers
• Quantification of free and total warfarin concentrations

Advantages of Our Warfarin Analysis Service

Constantly updated. We have a complete laboratory network and professional technicians to provide reliable plant targeted metabolomics service and real-time updates of industry knowledge.

One-stop service. Our laboratory can meet the needs of customers who require third-party testing services or wish to outsource their monitoring and analysis projects, and provide one-to-one one-stop service.

Professional and reliable. Creative Proteomics operates warfarin analysis under strict quality, health, safety and ethical control standards and has passed a number of professional certifications in the industry.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is up to 100%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

 Figure 1. Warfarin analysis service workflow.

Detection wavelength: 308nm

Mobile phase: Acetonitrile - water - glacial acetic acid

Linear range: 0.8-5.6 ug/ml, r=0.9996

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of warfarin
• Differential metabolites screening

Sample Requirements

• Serum/plasma ≥ 500 μl/sample
• Urine ≥ 1 ml/sample
• Tissue ≥ 200 mg/sample
• Cells: ≥ 1 × 10⁷/sample
• Feces: ≥ 500 mg/sample
• Drugs
• Others

We also accept other samples. Our technician will communicate with you on the exact amount of samples required.

Delivery

• Experimental procedure
• Parameters of UPLC and MS
• Raw data, chromatograms and mass spectra
• Metabolites quantification data
• Custom analysis report

Creative Proteomics is pleased to assist you with warfarin identification and quantitative analysis to aid in your research. If you need to analyze additional compounds, please contact us.