Ginsenoside Analysis Service

Ginseng is the dried root of araliaceae panax ginseng C.A.Mey, which is a valuable traditional Chinese medicine. The chemical composition of ginseng includes ginsenoside, sugars, amino acids, vitamins, proteins, polypeptides, organic acids, fat-soluble components and trace elements. Ginsenoside is the most important physiological active substances in the ginseng, with the function of regulating the central nervous system, improving cardiovascular and hematopoietic system, regulating the endocrine system and enhancing immunity. More than 50 kinds of monomer saponins have been found in ginseng and the analysis of ginsenoside has always been an active service project of Creative Proteomics. The different ginsenosides have their unique physiological functions, and the content of ginsenoside is an important index to evaluate the quality of ginseng and its products. Creative Proteomics provides the simple, rapid, accurate, affordable and highly automated analytical method and equipment to analyse ginsenosides. High performance liquid chromatography-mass spectrometry (HPLC-MS) can solve some of the problems associated with ginsenoside analysis. Improved extraction and purification steps can also increase the sensitivity of the analytical method.

Applications of Ginsenoside Analysis

• Qualitative analysis of the main types of ginsenosides
• Quantitative analysis of ginsenoside content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our Ginsenoside Analysis Service

Excellent ginsenoside qualitative and quantitative capabilities

Individualized experimental scheme

Full cycle quality control system

Professional data analysis skills

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and recovery rates consistently exceeding 85%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

 Figure 1. Ginsenoside analysis service workflow.

Detector: UV-Vis absorption detector and evaporative light scattering detector (ELSD)

Mobile phase: acetonitrile - water

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of ginsenoside
• Differential metabolites screening
• Optimal analyses

List of Detectable Ginsenoside at Creative Proteomics

Sample Requirements

1. Fresh ginseng tissues: >1 g. If possible. Samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

2. Product samples.

At least 3 biological replicates.

Delivery

• Experimental procedure
• Parameters of HPLC and MS
• Raw data, chromatograms and mass spectra
• Metabolites quantification data
• Custom analysis report

HPLC-MS-based profiling of ginsenoside enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC-MS services, Creative Proteomics has a proven track record supporting diverse ginsenoside detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.