Lysine Analysis Service
Online InquiryWhat is Lysine?
Lysine, an indispensable amino acid, holds a pivotal position in the intricate web of biological processes. As one of the nine essential amino acids that the human body cannot synthesize autonomously, lysine must be obtained through dietary sources. Its primary role lies in protein synthesis, where it serves as a fundamental building block. Lysine contributes significantly to the formation of collagen, a crucial structural protein, and plays a key role in the production of enzymes, hormones, and antibodies.
Beyond its role in protein synthesis, lysine is a central player in various metabolic pathways. It serves as a precursor for the biosynthesis of carnitine, an essential compound for energy production through fatty acid metabolism. Lysine is also involved in the synthesis of other important molecules, including acetylcholine, which plays a vital role in nerve transmission.
Lysine metabolism is a tightly regulated and dynamic process that involves a series of enzymatic reactions. One of the critical pathways is the saccharopine pathway, where lysine is converted into saccharopine by the enzyme lysine ketoglutarate reductase. Subsequent steps in this pathway lead to the formation of various metabolites, ultimately contributing to energy production and cellular homeostasis.
The metabolism of lysine is subject to intricate regulatory mechanisms to maintain balance within the cellular environment. Enzymes such as lysine decarboxylase and lysine-specific demethylase play crucial roles in modulating lysine levels and its diverse metabolic fates. Dysregulation of lysine metabolism has been linked to various pathological conditions, including metabolic disorders and certain types of cancer.
Lysine Analysis by Creative Proteomics
Creative Proteomics offers comprehensive lysine analysis services, employing cutting-edge techniques to provide detailed insights into lysine-containing proteins. Our services cover a range of analysis projects, ensuring accurate and reliable results for your research needs.
Identification and Quantification of Lysine Metabolites: Detection and measurement of metabolites derived from lysine metabolism, profiling changes under different biological or experimental conditions.
Metabolic Pathway Analysis: Evaluation of lysine metabolic pathways and their interconnected networks, providing a comprehensive understanding of the impact of lysine metabolism on cellular processes.
Metabolomic Profiling: Systematic analysis of the complete set of lysine-related metabolites in a given sample, identifying novel lysine-derived metabolites and their potential biological significance.
Pathway Enrichment Analysis: Statistical analysis to identify enriched pathways associated with lysine metabolites, integrating data to reveal global insights into lysine-related metabolic processes.
Lysine Quantification: Accurate measurement of lysine levels in biological samples, allowing for quantitative assessment across different conditions.
Lysine Post-Translational Modification (PTM) Analysis: Identification and characterization of various lysine PTMs, such as acetylation, methylation, and ubiquitination, to profile changes under specific biological contexts.
Lysine Residue Mapping: Mapping and identification of lysine residues within proteins, providing insights into their distribution and significance in protein structures.
Lysine-Containing Peptide Identification: High-throughput identification of peptides containing lysine residues using advanced mass spectrometry techniques.
Lysine Enrichment Strategies: Implementation of enrichment methods to isolate lysine-containing peptides or proteins, enhancing sensitivity and specificity in lysine analysis.
Customized Lysine Analysis Solutions: Tailored services to meet specific research objectives and project requirements, collaborating with researchers to design and execute customized lysine analysis projects.
Lysine Assay Platforms
Liquid Chromatography-Mass Spectrometry (LC-MS): Thermo Fisher Scientific Q Exactive series (e.g., Q Exactive HF-X, Q Exactive Plus)
Gas Chromatography-Mass Spectrometry (GC-MS): Agilent Technologies 5977B GC/MSD
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): AB Sciex Triple Quad 5500
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS): Bruker Daltonics MALDI TOF/TOF (e.g., UltrafleXtreme)
List of Detectable Lysine
Lysine | Acetyllysine | Methyllysine | Ubiquitinyllysine | Succinyllysine |
Malonyllysine | Glutaryllysine | Crotonyllysine | 2-Hydroxyisobutyryllysine | Butyryllysine |
3-Methylglutaryllysine | 3-Hydroxy-3-methylglutaryllysine | Beta-hydroxybutyryllysine | 2-Hydroxyglutarate | 3-Hydroxybutyryllysine |
4-Hydroxybutyryllysine | 2,3-Dihydroxybutyryllysine | Formyllysine | 3-Methyl-2-oxobutyryllysine | 3-Methylglutaconyllysine |
Sample Requirements of Lysine Analysis
Sample Type | Recommended Quantity |
---|---|
Serum/Plasma | 100-200 μL |
Whole Blood | 50-100 μL |
Tissue (e.g., liver, muscle) | 20-50 mg |
Cell Culture | 1 x 106 cells |
Urine | 1-2 mL |
Hair | 10-20 mg |