Valine is a branched chain non-polar α-amino acid containing five carbon atoms, which has a wide range of applications in human nutrition additives, feed additives, flavorings, pharmaceuticals, pesticides and health products. Creative Proteomics can provide high performance liquid chromatography - mass spectrometry (HPLC-MS) to detect amino acids, which can target and quantitatively detect valine and related valine metabolites in the constituent proteins, ensuring that it can provide customers with scientific, accurate and comprehensive valine analysis report.
Valine is a spinning compound and is classified as L-valine or D-valine. Chemically synthesized methionine is type L. L-valine is one of the 20 amino acids that make up proteins and is an essential amino acid for mammals and a sugar-generating amino acid. D-valine is present in some actinomycins such as valinomycin. In animals, L-Valine is readily absorbed by the intestinal wall and has twice the physiological effect of D-valine.
Animals cannot synthesize valine on their own and must consume it from food to meet their nutritional requirements. Meat, fish, dairy products and eggs are the richest food sources of valine. Valine promotes normal growth of the body, repairs tissues, regulates blood sugar, and provides needed energy. In addition, branched-chain amino acids are used as supplements for exercise training, cachexia syndrome (muscle atrophy) and strengthening muscle protein synthesis during aging. With the development of mass spectrometry-based amino acid metabolomics technology, valine metabolism characteristics in specific disease types can be determined to better guide researchers to use these valine and related metabolic pathways as targets for intervention and treatment research. The HPLC-MS method can realize the quantitative analysis of valine with high analytical speed and high analytical sensitivity.
Figure 1. BCAA synthesis occurs in plants, bacteria, and fungi. (Neinast 2019).
Applications of Valine Analysis
- Ensure food quality and safety
- Ensure the quality of biopharmaceuticals
- Reveal mechanisms of branched chain amino acid metabolism
- Animal feed improvement
- Quality control of valine samples
Advantages of Our Valine Analysis Service
First-class amino acid analysis experimental platform and rich analysis experience
Use stable and mature high-performance liquid chromatography analysis method
Simple and fast operation process and low price
Derivatives are stable and suitable for quantitative determination of amino acids
Great reproducibility, scientific and accurate measurement results of samples
In our operating technical process, the sample processing does not need derivatization, and can be directly injected for analysis, which simplifies the sample pretreatment process and reduces detection errors.
Figure 2. Valine analysis service workflow.
Quantitation method: standard curve method (external standard method or isotope-labeled internal standard method)
Quantitative limit: the lowest amount of accurate quantification of the sample, the S/N of the chromatographic peak is> 10:1
Analytical standards: HPLC ≥ 98%
The flow rate of the mobile phase in the column: reach 1-10ml/min
- Creation of calibration curve
- Obtaining the outflow curve
- Data analysis
- Tissue: 200 mg/sample
- Plasma ≥ 200 μL/sample
- Blood ≥ 200 μL/sample
- Drugs ≥ 0.2 g/sample
- Food products
We also accept other samples. Our technician will communicate with you on the exact amount of samples required.
- Experimental protocol
- Instrument parameters
- HPLC and MS raw data analysis report and data quality checks
- Valine quantitative analysis report
- Custom analysis report
The analysis of valine and related valine metabolites based on HPLC-MS can ensure fast, high sensitivity, high accuracy and complete valine quantitative analysis report. Creative Proteomics can provide a first-class experimental platform and stable and mature experimental technology, experienced researchers to help customers deal with diversified samples, and can provide you with customized services.
- Neinast M, Murashige D, Arany Z. Branched Chain Amino Acids. Annual review of physiology. 2019;81:139-164.
- Mráz J, Hanzlíková I, Dušková Š, et al. Determination of N-(2-hydroxyethyl)valine in globin of ethylene oxide-exposed workers using total acidic hydrolysis and HPLC-ESI-MS2. Toxicol Lett. 2018;298:76-80.
For Research Use Only. Not for use in diagnostic procedures.