Arginine Analysis Service

What is Arginine?

Arginine is an amino acid compound that participates in the ornithine cycle in the human body, promotes the formation of urea, and converts the ammonia produced in the human body into non-toxic urea through the ornithine cycle. Arginine has three tightly bound nitrogen atoms in its side chain. It is an extremely alkaline amino acid with a pH of up to 12.5. Arginine metabolism ultimately produces a variety of biochemical products, including nitric oxide, urea, creatine, polyamines, proline, glutamate, agmatine, and homoarginine. The change of arginine concentration can also regulate cell metabolism and function through a variety of arginine sensors. Arginine forms an electrostatic complex with aspartic acid or glutamic acid to strengthen the tertiary structure of the protein. It also interacts with acidic groups attached to proteins, such as the phosphate groups of nucleic acids, thereby regulating the function of proteins, which are essential amino acids for maintaining growth and development. Arginine can be used in biochemical research. For example, as an intercellular messenger in the nitric oxide pathway, it plays an important role in the regulation of the nervous system. It is also used as a nutritional supplement, flavoring agent and food flavor in the food field.

Creative Proteomics offers a rapid, highly selective high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method to obtain the content of arginine and its derivatives and provide customers with detailed analytical reports. The method is sensitive, fast, reproducible and accurate, helping to accelerate the exploration of the mechanism of action of arginine and its derivatives and the application of its medicinal value to our customers.

Figure 1. Pathways of arginine catabolism. (Morris 2016).

Applications of Arginine Analysis

• Accurately quantify the amount of arginine in various biological samples, such as plasma, urine and tissue, in clinical and research settings.
• Analyze arginine metabolites and identify new metabolites produced in different biological systems to gain insight into the biological function of arginine and its role in disease processes.
• To study the pharmacokinetics of arginine-based drugs. such as arginine pressin, which is used to treat diabetes mellitus and septic shock. Provide information on the absorption, distribution, metabolism and excretion of drugs.
• Analyze the arginine content of foods and supplements, such as protein powders and energy drinks, to ensure product quality and safety.

Advantages of Our Arginine Analysis Service

• Efficient and accurate analysis report
• Professional research team and stable and reliable analysis technology
• Fast turnaround time and competitive price
• One-stop platform for testing services to meet diverse testing needs
• HPLC-MS platform for accurate analysis of arginine and its metabolites

Service Workflow

We can use the HPLC-MS/MS method to achieve the high-throughput detection and analysis requirements of the samples, and can quantify the arginine in a variety of samples and obtain detailed analysis reports.

Figure 2. Arginine analysis service workflow.

Detection method: HPLC-MS/MS

Mobile phase: use ammonium dihydrogen phosphate solution-acetonitrile as mobile phase

Elution mode: linear gradient elution

Detection wavelength: 206 nm

Injection volume: 25 μl

Flow rate: 1.0 ml/min

Analysis content:

• Stability test
• Precision test
• Repeatability test
• Sample recovery test
• Production of standard straight lines
• Optimization of HPLC chromatographic analysis conditions
• Recovery rate experiment
• Chromatographic separation and screening
• Chromatogram raw image and data collection
• Identification and content determination of arginine and its derivatives

Sample Preparation

We can accept all kinds of specimens, such as serum, plasma, cell culture supernatant, urine, animal and plant tissues, etc. Please contact us for details.

Delivery

• Detailed experimental steps
• Sample pretreatment analysis
• Chromatographic separation conditions and peak area calculation
• Optimal parameter analysis of HPLC equipment
• Chromatogram raw image and data analysis report
• Analysis report on identification and content determination of arginine and its derivatives
• Customized analysis report

We can use HPLC-MS/MS technology to achieve high-throughput and accurate identification and quantitative analysis of arginine and its derivatives, which will help researchers realize the comprehensive application of arginine in related fields such as food and medicine. Creative Proteomics has relatively reliable bioinformatics analysis resources in the metabolic analysis of amino acids and their derivatives, which can meet your diversified testing needs, and we will wholeheartedly provide you with customized services.

References

1. Morris SM Jr. Arginine Metabolism Revisited. The Journal of Nutrition. 2016;146(12):2579S-2586S.
2. Ridwan R, Abdul Razak H R, et al. Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons. International Journal of Analytical Chemistry. 2018.