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Thiamine Metabolites and Derivatives Analysis

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What is Thiamine?

Thiamine, often known as vitamin B1, is a water-soluble B-complex vitamin. It is received through nutrition and plays an important function in cellular metabolism. Thiamine is largely engaged in carbohydrate energy conversion, acting as a cofactor for various enzymes in critical metabolic processes. It is required for the normal function of organs and tissues such as the brain and nervous system.

Thiamine Metabolites and Derivatives

Thiamine goes through a complicated metabolic process in the body. Thiamine is phosphorylated into its active form, thiamine diphosphate (TDP), in the intestines by the activity of thiamine pyrophosphokinase. TDP is a cofactor for various carbohydrate metabolism enzymes, including pyruvate dehydrogenase and -ketoglutarate dehydrogenase.

Thiamine monophosphate kinase is able to convert thiamine into thiamine monophosphate (TMP). TDP and other thiamine derivatives can only be made using TMP. Additionally, thiamine can be esterified to produce a variety of thiamine esters. Cellular signaling and energy metabolism are linked to thiamine triphosphate (TTP) and thiamine tetraphosphate (TTPP) esters.

Thiamine derivatives are substances that result from the modification or combination of thiamine molecules with other molecules. These derivatives may have different roles and qualities than thiamine itself. Thiamine pyrophosphate (TPP), commonly known as cocarboxylase, is a well-known thiamine derivative. TPP is a cofactor for various enzymes involved in energy metabolism, including pyruvate dehydrogenase and -ketoglutarate dehydrogenase complexes.

Thiamine Analysis Platform at Creative Proteomics

The overall workflow of thiamine metabolismFigure 1. The overall workflow of thiamine metabolism

At Creative Proteomics, we offer advanced and comprehensive analysis platforms for thiamine metabolites and derivatives.

The analysis poses several challenges due to their low concentrations in biological samples and the need to differentiate them from other compounds. Liquid chromatography-mass spectrometry (LC-MS) has emerged as a powerful analytical platform for the analysis of thiamine and its derivatives. LC-MS combines the separation capabilities of liquid chromatography with the detection and identification power of mass spectrometry, offering high sensitivity and selectivity.

For targeted analysis of thiamine metabolites and derivatives, we employ multiple reaction monitoring (MRM) mode in our LC-MS analysis. MRM allows for the specific detection and quantification of thiamine and its derivatives by monitoring precursor-to-product ion transitions. This mode offers enhanced sensitivity, selectivity, and reproducibility, ensuring reliable and accurate results.

We adhere to strict quality control measures and follow standardized protocols to ensure the accuracy and reliability of our analysis. Our dedicated quality assurance team monitors each step of the analysis process to guarantee the highest level of data integrity.

List of Partial Detectable Thiamine Metabolites and Its Derivatives

ThiamineThiamine diphosphate (TDP)Thiamine monophosphate (TMP)Thiamine triphosphate (TTP)
Thiamine tetraphosphate (TTPP)Thiamine pentaphosphate (TPPP)Thiamine triphosphate-adenosine (TPP-Ad)Adenosine thiamine triphosphate (ATP)
Adenosine thiamine triphosphate (ATP)Thiamine disulfide (TDS)Thiamine borateThiamine propyldisulfide
Thiamine propyl disulfide monophosphate


Sample Requirements of Thiamine Analysis

Sample TypeSample VolumeSample Matrix
Whole Blood100-500 μLAnticoagulant: EDTA, heparin, or citrate
Plasma100-500 μLAnticoagulant: EDTA, heparin, or citrate
Serum100-500 μLGel-separator tube
Urine100-500 μLMidstream or random collection
Tissue Homogenate50-100 mgFrozen or fresh tissue
Food SamplesVariableDepends on the type and preparation
For Research Use Only. Not for use in diagnostic procedures.
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