Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Thiamine Analysis Service

Thiamine Analysis Service

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Creative Proteomics is a pioneer in metabolic analysis service and provides reliable, professional and cost-effective thiamine metabolism analysis service in animal. We leverage our rich experience, professional expertise, and advanced technologies to speed up your scientific purposes of thiamine metabolism analysis in animal.

Overview

Thiamine was the first B vitamin to have been identified, thus also known as vitamin B1. Most animals lack privilege of its in vivo biosynthesis of thiamine and obtained in certain amounts from dietary supplement as an essential micronutrient. Food sources of thiamine contain whole grains, some meats and fish. In contrast, ruminal can synthesised almost 90 % of thiamine via microorganisms. In the body, thiamine occurs as free thiamine and various phosphorylated forms: thiamine monophosphate, thiamine triphosphate and thiamine pyrophosphate. The hydroxyl group of thiamine in thiamine pyrophosphate is replaced by a diphosphate ester group. Thiamine pyrophosphate is the active form of thiamine and it serves as a cofactor for several enzymes involved in energy metabolism. These enzymes include the mitochondrial pyruvate dehydrogenase, the a-ketoglutarate dehydrogenase complexes and the cytosolic transketolase, all of which participate in carbohydrate catabolism by regulating the Krebs cycle. Deficiency of thiamine leads to a reduction in the activity of these enzymes, albeit its effects on the different enzymes is different. The aberrations of thiamine metabolism are closely associated with various human diseases like Alzheimer's disease and depression. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of thiamine metabolism related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective thiamine metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Thiamine Analysis Service

  • Identifying novel biomarkers of diseases
  • Improving diagnose diseases
  • Accelerating development of novel drugs
  • Gaining new mechanism of diseases

Advantages of Our Thiamine Analysis Service

  • Constantly optimized protocol and analytical software
  • Professional experiment design
  • Quick turnaround time
  • High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides thiamine metabolism analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography- mass spectrometry (HPLC-MS) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

The overall workflow of thiamine metabolismFigure 1. The overall workflow of thiamine metabolism

List of Partial Detectable Thiamine Metabolism Related Metabolites at Creative Proteomics

Thiaminethiamine triphosphate2-methyl-4-amino-5-pyrimidine carboxylic acid
Thiamine monophosphate4-methyl-thiazole-5-acetic acid 
Thiamine pyrophosphateThiamine acetic acid 

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Tissue: 200mg/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample
    Shipment condition: dry ice

Report Delivery

  • Experimental protocols
  • Instrumental factors of HPLC
  • The raw data files of HPLC and the summary of HPLC data quality
  • Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of thiamine metabolism related metabolites, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored thiamine metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

  1. Breves G, Brandt M, Hoeller H. et al. Flow of thiamin to the duodenum in dairy cows fed different rations. J Agr Sci.1981. 96: 587–591.
  2. Dhir Shibani, Tarasenko Maya, Napoli Eleonora. et al. Neurological, Psychiatric, and Biochemical Aspects of Thiamine Deficiency in Children and Adults. Front Psychiatry. 2019.10:207.
For Research Use Only. Not for use in diagnostic procedures.

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