Metabolomics Creative Proteomics

Riboflavin Analysis Service

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What is Riboflavin and What Does it Do?

Riboflavin (Vitamin B2) a water-soluble vitamin, is an essential nutrient in vertebrates, hence adequate dietary intake is necessary. Endogenous synthesis of riboflavin by microflora in the large intestine might contribute to a lesser extent. Riboflavin has a role in various metabolic pathways, serving mainly as an integral component of its crucial biologically active forms (flavocoenzymes flavin adenine dinucleotide and flavin mononucleotide). These flavocoenzymes ensure the functionality of tremendous flavoproteins including oxidases, dehydrogenases, monooxygenases and reductases, which play crucial roles in a variety of cellular function including mitochondrial electron transport chain, β-oxidation of fatty acids, redox homeostasis, citric acid cycle, apoptosis and more. Riboflavin is associated with the treatment of cancer, heart disease, high blood pressure, burns, arthritis and other diseases. In plant research, riboflavin induces protein kinase gene expression and disease resistance and initiates molecular responses in plant growth signaling pathways.

Creative Proteomics utilizes UPLC-MS technology platform to enable qualitative and quantitative riboflavin analysis services in different samples.

The Main Applications of Riboflavin Analysis Service

Food and nutrition: Determining the amount of riboflavin in foods and supplements.

Pharmaceuticals: Determining the quality and purity of riboflavin used as an active ingredient in pharmaceuticals and supplements, monitoring the stability of riboflavin in pharmaceutical forms.

Basic Research: Measuring riboflavin levels in blood, urine, and other biological fluids.

Environmental Monitoring: Assess the impact of environmental factors such as light and oxygen on riboflavin degradation in water and soil.

Advantages of Our Riboflavin Analysis Service

  • Constantly optimized protocol and analytical software
  • Professional experiment design
  • Quick turnaround time
  • High accuracy, specificity, and sensitivity

Service Workflow

The overall workflow of riboflavin metabolism

List of Partial Detectable Riboflavin Related Metabolites at Creative Proteomics

Metabolite NameMolecular Formula
Riboflavin C17H20N4O6
Flavin adenine dinucleotide (FAD) C27H33N9O15P2
Flavin mononucleotide (FMN) C17H21N4O9P
Lumichrome C15H12N4O2
Lumiflavin C17H13N3O2
Riboflavin 5'-phosphate (FMN) C17H21N4O9P
Riboflavin 5'-adenosine monophosphate (FMN) C27H31N9O14P2
Riboflavin tetraacetate C25H28N4O10
Riboflavin 5',6'-oxide C17H18N4O6
7,8-Dimethyl-10-ribityllumazine C17H23N4O2
6,7-Dimethyl-8-ribityllumazine C17H23N4O2
5-Amino-6-(ribosylamino)uracil C12H18N4O7

Sample Requirements

  • Plant tissues: 400mg/sample
  • Animal tissues: 200mg/sample
  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample

Shipment condition: dry ice

Report Delivery

  • Experimental procedures
  • The parameters of liquid chromatograph and mass spectrometer
  • HPLC and MS raw data files
  • Metabolite quantitative data


  1. Barile Maria, Giancaspero Teresa Anna, Leone Piero. et al. Riboflavin transport and metabolism in humans. J Inherit Metab Dis. 2016.39:545-557.
  2. Mosegaard Signe, Dipace Graziana, Bross Peter. et al. Riboflavin Deficiency-Implications for General Human Health and Inborn Errors of Metabolism. Int J Mol Sci. 2020.21:3847.
For Research Use Only. Not for use in diagnostic procedures.


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