What is Coenzyme A?
Coenzyme A, abbreviated as CoA, plays a crucial role in various vital biochemical reactions. It consists of three main components: a beta-mercaptoethylamine arm, a pantothenic acid arm, and a terminal adenosine diphosphate (ADP) arm. These structural elements make CoA an indispensable molecule in energy metabolism since it is essential for both the creation and breakdown of fatty acids and the citric acid cycle.
The primary function of CoA revolves around its role as a carrier for acyl groups. It acts like a "molecular handle" that enables enzymes to manipulate and transport acyl groups, thereby facilitating reactions in different metabolic pathways. The acyl group forms a high-energy thioester bond with the thiol group of CoA, allowing it to participate in various metabolic processes.
The metabolism of Coenzyme A is a complex network of enzymatic reactions involving its synthesis and degradation. These processes are meticulously regulated to maintain the balance of energy and cellular equilibrium. It's crucial to quantitatively assess CoA levels in diverse biological samples to gain insights into metabolic conditions and related disorders.
Specific Coenzyme A Analysis Services Offered by Creative Proteomics
Creative Proteomics offers a range of CoA analysis services to help researchers and industries understand and manipulate CoA metabolism. These services are based on cutting-edge analytical techniques, particularly mass spectrometry, which provides high sensitivity and specificity in CoA analysis.
Coenzyme A Quantification: We utilize high-precision equipment, such as the Agilent 1290 Infinity II LC System coupled with the Agilent 6550 iFunnel Q-TOF MS, for the quantification of CoA content. This aids in determining the concentration of CoA in different biological samples.
Workflow of coenzyme A metabolites analysis
Coenzyme A Metabolic Pathway Analysis: We investigate the role of CoA in metabolic pathways using advanced equipment like the Thermo Fisher Scientific TRACE™ 1310 GC coupled with the Thermo Fisher Scientific Q Exactive GC Hybrid Quadrupole-Orbitrap MS. This analysis includes the study of CoA's involvement in pathways such as fatty acid synthesis and the citric acid cycle.
Analysis of Coenzyme A Derivatives: We perform precise concentration measurements of CoA derivatives, including Acetyl-CoA, Malonyl-CoA, and Palmitoyl-CoA, using liquid chromatography-mass spectrometry (LC-MS) equipment such as the Waters Acquity UPLC System coupled with the Waters Xevo G2-XS QTOF MS.
Structural Identification of Coenzyme A: Using nuclear magnetic resonance (NMR) technology, such as the Bruker Avance III HD 600 MHz NMR, we determine the structural aspects of CoA, facilitating a deeper understanding of its chemical properties within different metabolic pathways.
Correlation Analysis of Coenzyme A and Related Substances: We employ advanced analytical tools, such as the Agilent 1290 Infinity II LC System coupled with the Agilent 6550 iFunnel Q-TOF MS, to assess the correlations between CoA and other metabolites, uncovering potential biological and medical associations.
Advantages of Our Coenzyme A Analysis Service
- Isotope internal standard correction. Absolute quantification by external standard method. Absolute concentration data available.
- Provide PCA, PLS-DA, OPLS-DA, unidimensional test, Random Forest and SVM screening, ROC analysis results
- Adopt strict quality control system to ensure data reliability
- Professional and complete after-sales service
List of Coenzyme A and Its Derivatives for Analysis
| Compound | Category |
|---|
| Coenzyme A | CoA and Analogue |
| Coenzyme A-SH (CoASH) | CoA and Analogue |
| Acetyl-CoA | CoA and Analogue |
| Malonyl-CoA | CoA and Analogue |
| Succinyl-CoA | CoA and Analogue |
| Glutaryl-CoA | CoA and Analogue |
| 3-Hydroxy-3-Methylglutaryl-CoA | CoA and Analogue |
| 3-Mercaptopropionylglycine | CoA and Analogue |
| 3'-Phosphoadenylylsulfate | CoA and Analogue |
| Compound | Category |
|---|
| Butyryl-CoA | CoA Derivatives |
| Cerotyl-CoA | CoA Derivatives |
| Behenyl-CoA | CoA Derivatives |
| Arachidonyl-CoA | CoA Derivatives |
| Linoleoyl-CoA | CoA Derivatives |
| Lignoceryl-CoA | CoA Derivatives |
| Oleoyl-CoA | CoA Derivatives |
| Palmitoleoyl-CoA | CoA Derivatives |
| Isovaleryl-CoA | CoA Derivatives |
| Stearoyl-CoA | CoA Derivatives |
Sample Requirements for Coenzyme A Assay
| Sample Type | Sample Volume | Storage Conditions | Special Instructions |
|---|
| Serum or Plasma | 0.5 mL | -80°C | Avoid repeated freeze-thaw cycles. |
| Tissue (e.g., Liver) | 20 mg | -80°C | Homogenize the tissue thoroughly. |
| Cells (e.g., Cultured) | 2 x 106 cells | -80°C | Harvest and store cells in a suitable buffer. |
| Urine | 1 mL | -20°C | Collect in a clean, sterile container. |
| Whole Blood | 0.5 mL | -80°C | Use an anticoagulant (e.g., EDTA) if required. |
| Cerebrospinal Fluid (CSF) | 0.2 mL | -80°C | Handle samples with care to avoid contamination. |
| Saliva | 1 mL | -20°C | Collect using a sterile collection tube. |
| Feces | 2 g | -80°C | Freeze samples immediately after collection. |
Report Delivery
- Experimental procedures
- The parameters of liquid chromatograph and mass spectrometer
- Purity analysis report
- MS raw data files and MS data quality inspection
- Absolute quantitative analysis data
Based on advanced mass spectrometry platform, professional bioinformatics software and experienced technicians and scientists, Creative Proteomics provides customers with coenzyme A detection and analysis services to accelerate your scientific research. If you want to analyze other compounds, please contact us.
For Research Use Only. Not for use in diagnostic procedures.
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