Ubiquinone And Other Terpenoid-Quinone Biosynthesis Service

Overview

Ubiquinone (UQ), also known as coenzyme Q (CoQ), is a redox-active lipid present in all cellular membranes in which it acts in a range of biological processes.UQ is a benzoquinone linked to a hydrophobic poly-isoprenoid tail. Therefore, the overall architecture of CoQ biosynthesis includes a long polyisoprenoid lipid tail coupling to a benzenoid precursor, and then benzenoid ring is further modified through consecutive steps to produce the final product. All eukaryotic cells can synthesize their own UQ isoprene units (CoQ9 or CoQ10). The pathogenesis of CoQ10 deficiency involves two main aspects: reduced ATP production and reactive oxygen species production, which is closely associated with human diseases like cancer. The terpenoids are the largest class of plant secondary metabolites, deriving from the 5-carbon compound isoprene. The starting primary metabolite for terpenoids synthesis is acetyl-CoA that goes through a series of reactions to condense three molecules of acetyl-CoA to mevalonic acid. The six-carbon mevalonic acid is the immediate precursor of the basic 5-carbon building blocks like isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), which are key players in the terpenoids biosynthesis. A sufficient supply of IPP and DMAPP is the basic premise for terpenoids biosynthesis. Quinones are oxidized derivatives of aromatic compounds and are readily made from reactive aromatic compounds with electron-donating substituents such as phenols and catechols. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of ubiquinone and other terpenoid-quinone biosynthesis related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective ubiquinone and other terpenoid-quinone biosynthesis related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Ubiquinone And Other Terpenoid-Quinone Biosynthesis Service

• Identifying novel biomarkers of diseases
• Improving diagnose diseases
• Accelerating development of novel drugs
• Gaining new mechanism of diseases

Advantages of Our Ubiquinone And Other Terpenoid-Quinone Biosynthesis Service

• Constantly optimized protocol and analytical software
• Professional experiment design
• Quick turnaround time
• High accuracy, specificity, and sensitivity.

Service Workflow

Creative Proteomics provides ubiquinone and other terpenoid-quinone biosynthesis analysis service in a reliable and effective manner, based on our cutting-edge HPLC-MS platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of lipoic acid metabolism

List of Partial Detectable Ubiquinone And Other Terpenoid-Quinone Biosynthesis Related Metabolites at Creative Proteomics

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample

Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of ubiquinone and other terpenoid-quinone biosynthesis related metabolites, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored ubiquinone and other terpenoid-quinone biosynthesis analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Wang Y, Hekimi S. The Complexity of Making Ubiquinone. Trends Endocrinol. Metab. 2019. 30:929–943.
2. Formighieri C, Melis A. Carbon partitioning to the terpenoid biosynthetic pathway enables heterologous β-phellandrene production in Escherichia coli cultures. Arch Microbiol. 2014.196:853–861.