Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

One Carbon Pool By Folate Service

One Carbon Pool By Folate Service

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Overview

As a member of enzyme cofactors, folate chemically activates one-carbon units for biosynthesis of formate, methanol and formaldehyde (Figure 1). One-carbon metabolism widely exists in the cytoplasm, mitochondria, and nucleus (Figure 1). They are the interdependent and indispensable biosynthetic pathways for polyamine synthesis and methylation reactions of a large variety of molecules such as neurotransmitters, phospholipids, proteins and numerous other small molecules. In the mitochondria, the one-carbon metabolism is also required for numerous reactions like the interconversion of glycine and serine, the metabolism of choline, purines and the synthesis of formylated methionyl-tRNA. Additionally, the one-carbon metabolism in the mitochondria provides most of the one-carbon units used for metabolism in cytoplasm. Altogether, folate molecules function as carriers for one-carbon units, allowing them to be manipulated and assembled in support of metabolic processes. Impairments in folate-mediated one-carbon metabolism are closely associated with various human diseases like Alzheimer and cancer. Scientists at Creative Proteomics take advantage of highly quantitative methods with high-performance liquid chromatography (HPLC) for the determination of one carbon pool by folate related metabolites levels in various samples. Furthermore, we provide reliable, rapid and cost-effective one carbon pool by folate related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The overview of folate-mediated one carbon metabolismFigure 1. The overview of folate-mediated one carbon metabolism

The Main Applications of One Carbon Pool By Folate Service

  • Identifying novel biomarkers of diseases
  • Improving diagnose diseases
  • Accelerating development of novel drugs
  • Gaining new mechanism of diseases

Advantages of Our One Carbon Pool By Folate Service

  • Constantly optimized protocol and analytical software
  • Professional experiment design
  • Quick turnaround time
  • High accuracy, specificity, and sensitivity.

Service Workflow

Creative Proteomics provides one carbon pool by folate analysis service in a reliable and effective manner, based on our cutting-edge HPLC-MS platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC-MS data analysis and bioinformatics analysis (Figure 2). Our service will be tailored to specific samples and needs for optimal results.

The overall workflow of one carbon pool by folateFigure 2. The overall workflow of one carbon pool by folate

List of Partial Detectable One Carbon Pool By Folate Related Metabolites at Creative Proteomics

Folic acidTetrahydrofolate5-Formiminotetrahydrofolate
Folinic acid10-Formyltetrahydrofolate5,10-Methylenetetrahydrofolate
Dihydrofolate5,10-Methenyltetrahydrofolate5-Methyltetrahydrofolate

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

  • Blood/plasma: 500ul/sample
  • Urine: 1ml/sample
  • Tissue: 200mg/sample
  • Cells: 1x107/sample
  • Feces: 500mg/sample

Shipment condition: dry ice

Report Delivery

  • Experimental protocols
  • Instrumental factors of HPLC
  • The raw data files of HPLC and the summary of HPLC data quality
  • Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of one carbon pool by folate related metabolites, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored one carbon pool by folate analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

  1. Ducker Gregory S, Rabinowitz Joshua D. One-Carbon Metabolism in Health and Disease.Cell Metab. 2017.25:27-42.
  2. Newman Alice C, Maddocks Oliver D K. One-carbon metabolism in cancer. Br J Cancer. 2017. 116:1499-1504.
For Research Use Only. Not for use in diagnostic procedures.

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