Pantothenate (vitamin B5) is the key precursor for the biosynthesis of CoA, an important cofactor involved in a variety of metabolic reactions including the citrate cycle and fatty acid biosynthesis. Pantothenate synthesis happens in plants, fungi and some bacteria, whereas animals salvage pantothenate from diet. In all living organisms, CoA is synthesized via a five-step process, which requires pantothenate and ATP as well as cysteine. The five-step enzymatic reaction is catalyzed by pantothenic acid kinase, phosphopantetheinylcysteine synthetase, phosphopantetheinylcysteine decarboxylase, phosphopantetheinylmercaptoethylamine adenosyltransferase, and dephosphorylated coenzyme A kinase, in that order. In addition, CoA can be synthesized via alternate routes when intracellular CoA level are reduced and the in vivo pathway is impaired. The aberrant pantothenate and CoA biosynthesis therefore leads to human diseases like neurodegenerative diseases and cancers.
Creative Proteomics utilizes high performance liquid chromatography (HPLC) or high performance liquid chromatography-mass spectrometry (LC-MS) to determine levels of pantothenate and coenzyme a biosynthesis-related metabolites in a variety of samples to accelerate your research project, from disease diagnosis to new insights into disease mechanisms or treatments.
The Main Applications of Pantothenate And CoA Biosynthesis Service
- Identifying novel biomarkers of diseases
- Improving diagnose diseases
- Accelerating development of novel drugs
- Gaining new mechanism of diseases
Advantages of Our Pantothenate And CoA Biosynthesis Service
- Constantly optimized protocol and analytical software
- Professional experiment design
- Quick turnaround time
- High accuracy, specificity, and sensitivity.
Creative Proteomics is based on our cutting-edge HPLC and HPLC-MS platforms to provide the analysis of metabolites during pantothenate and CoA biosynthesis in a reliable and efficient manner. The experimental process consists of four main steps: sample collection, metabolite extraction, data analysis and bioinformatics analysis. We will tailor our services to specific samples and needs in order to obtain optimal results.
The overall workflow of pantothenate and CoA biosynthesis metabolites analysis
List of Partial Detectable Pantothenate And CoA Biosynthesis Related Metabolites at Creative Proteomics
|Pantothenate||Phosphopantetheine||2-Amino-3-methylbutyric acid||3-Aminopropionic acid|
|Pantoic acid||Dephosphocoenzyme A||2-Aminosuccinic acid||2-Dehydropantoate|
|Coenzyme A||3-Methyl-2-oxobutanoic acid||D-4'-Phosphopantothenate||(R)-4'-Phosphopantothenoyl-L-cysteine|
Creative Proteomics can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of samples:
- Blood/plasma: 500ul/sample
- Urine: 1ml/sample
- Tissue: 200mg/sample
- Cells: 1x107/sample
- Feces: 500mg/sample
At least 6 biological replicates per group for cellular/microbial samples.
At least 10 biological replicates per group for model animal/plant samples.
Clinical samples generally have at least 50 biological replicates per group.
Shipment condition: dry ice
- Experimental procedures
- The parameters of liquid chromatograph and mass spectrometer
- Purity analysis report
- MS raw data files and MS data quality inspection
- Absolute quantitative analysis data
Based on advanced mass spectrometry platform, professional bioinformatics software and experienced technicians and scientists, Creative Proteomics provides customers with rapid experimental process and easy-to-read reports for pantothenate and coenzyme a biosynthesis-related metabolite detection and analysis services to accelerate your scientific research.
- Czumaj Aleksandra, Szrok-Jurga Sylwia, Hebanowska Areta. et al. The Pathophysiological Role of CoA. Int J Mol Sci. 2020.21:9057.
- Leonardi Roberta, Jackowski Suzanne. Biosynthesis of Pantothenic Acid and Coenzyme A. EcoSal Plus. 2007.2:10.
For Research Use Only. Not for use in diagnostic procedures.