Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Melatonin Analysis Service

Melatonin Analysis Service

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Our melatonin (MT) profiling service allows you to detect and measure MT and its metabolites from single plant tissue samples. Whether you are working to improve crop productivity, uncover the biosynthesis, signaling and action of MT, Creative Proteomics can help you comprehensively understand MT involvement in a plant’s biological processes.

Overview

Recent years, there has been a growing attention and interest in studies on MT (N-acetyl-5-methoxy-tryptamine) in plants. MT is an indoleamine neurohormone that has been identified in more than 140 plant species in the past 20 years. Studies have shown that MT is implicated in almost all of the plant growth and development stages. Additionally, MT is reported to effectively protect plant tissues via responses to a variety of abiotic and biotic stresses, including drought, salt, cold, heat, and pathogen infection. Accurate and sensitive detection and quantification of endogenous MT enable us to better understand its physiological functions, biological synthesis, metabolic pathways, and regulatory networks. Ultra-performance liquid chromatography (UPLC) coupled with mass spectrometry (MS) has been proved as a reliable approach for analyzing MT content in plants because of its high sensitivity, reproducibility and accuracy.

MTsynthesis pathwayFigure 1. MTsynthesis pathway (Arnao & Hernández‐Ruiz 2020).

Applications of MT Analysis

  • Revealing the roles of MT in plant growth and development stages
  • Elucidating the mechanisms of MT biosynthesis and metabolic pathways
  • Understanding MT signal transduction and the regulatory networks
  • Promoting the application of MT in sustainable agriculture

Advantages of Our MT Analysis Service

High recovery rates and effectiveness

Generating accurate, sensitive, reproducible data

High-resolution LC-MS platform using MRM acquisition mode

Flexibility to handle a variety of sample types

Decades of experience in plant hormone analysis

Fast turnaround time and comparable price

Service Workflow

Creative Proteomics offers a highly sensitive and selective method for detecting and quantifying MT in plants using UPLC-MS/MS. We are experienced in dealing with a variety of sample types, and achieve high sensitivity and specificity due to reduced background noise, without homogeneity between samples.

Service Workflow

Quantitation method: external standard method

Analysis content:

  • Raw data preprocessing
  • Absolute quantitation of MT
  • Differential metabolites screening
  • Partial least squares-discriminant analysis (PLS-DA) and principle component analysis (PCA)

List of Detectable MT at Creative Proteomics

Detectable MTCASQuantitation Method
MT73-31-4External standard method

Sample Requirements

Fresh plant tissues > 1 g. Please provide fresh plant tissues, if possible. Samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

At least 3 biological replicates.

Delivery

  • Experimental procedure
  • Parameters of UPLC-MS
  • Extracted mitochondria and purified protein sample
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • MT quantification data
  • Custom data analysis report

Creative Proteomics is specialized in offering a battery of plant hormone analysis services that are powered by state-of-the-art gas chromatography coupled to mass spectrometry (GC-MS/MS) or UPLC-MS/MS, contributing to elucidating the functions, biological synthesis, metabolism, and networks of phytohormones.

References

  1. Ye T, Hao Y H, Yu L, et al. A simple, rapid method for determination of melatonin in plant tissues by UPLC coupled with high resolution orbitrap mass spectrometry. Frontiers in plant science, 2017, 8: 64.
  2. Erland L A E, Chattopadhyay A, Jones A M P, et al. Melatonin in plants and plant culture systems: Variability, stability and efficient quantification. Frontiers in plant science, 2016, 7: 1721.
  3. Arnao M B, Hernández‐Ruiz J. Melatonin as a regulatory hub of plant hormone levels and action in stress situations. Plant Biology, 2020.
For Research Use Only. Not for use in diagnostic procedures.

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