Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Melatonin Analysis Service

Melatonin Analysis Service

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Melatonin (N-acetyl-5-methoxy-tryptamin) is an indoleamine neurohormone that has been identified in more than 140 plant species in the past 20 years. Studies have shown that melatonin is implicated in almost all of the plant growth and development stages. Additionally, melatonin is reported to effectively protect plant tissues via responses to a variety of abiotic and biotic stresses, including drought, salt, cold, heat, and pathogen infection. Accurate and sensitive detection and quantification of endogenous melatonin enable us to better understand its physiological functions, biological synthesis, metabolic pathways, and regulatory networks. Ultra-performance liquid chromatography (UPLC) coupled with mass spectrometry (MS) has been proved as a reliable approach for analyzing melatonin content in plants because of its high sensitivity, reproducibility and accuracy.

Our melatonin profiling service allows you to detect and measure melatonin and its metabolites from single plant tissue samples. Whether you are working to improve crop productivity, uncover the biosynthesis, signaling and action of melatonin, Creative Proteomics can help you comprehensively understand melatonin involvement in a plant's biological processes.

MTsynthesis pathwayFigure 1. MTsynthesis pathway (Arnao & Hernández‐Ruiz 2020).

Applications of Melatonin Analysis

  • Revealing the roles of melatonin in plant growth and development stages
  • Elucidating the mechanisms of melatonin biosynthesis and metabolic pathways
  • Understanding melatonin signal transduction and the regulatory networks
  • Promoting the application of melatonin in sustainable agriculture

Advantages of Our Melatonin Analysis Service

High recovery rates and effectiveness

Generating accurate, sensitive, reproducible data

High-resolution LC-MS platform using MRM acquisition mode

Flexibility to handle a variety of sample types

Decades of experience in plant hormone analysis

Fast turnaround time and comparable price

Service Workflow

Creative Proteomics offers a highly sensitive and selective method for detecting and quantifying melatonin in plants using UPLC-MS/MS. We are experienced in dealing with a variety of sample types, and achieve high sensitivity and specificity due to reduced background noise, without homogeneity between samples.

Service Workflow

Quantitation method: external standard method

Analysis content:

  • Raw data preprocessing
  • Absolute quantitation of melatonin
  • Differential metabolites screening
  • Partial least squares-discriminant analysis (PLS-DA) and principle component analysis (PCA)

Sample Requirements

Fresh plant tissues > 1 g. Please provide fresh plant tissues, if possible. Samples should be frozen in liquid nitrogen right after collection, and then transferred to -80°C for storage.

At least 3 biological replicates.

Delivery

  • Experimental procedure
  • Parameters of UPLC-MS
  • MS raw data files and MS data quality checks
  • Melatonin quantification data
  • Custom data analysis report

Creative Proteomics is specialized in offering a battery of plant hormone analysis services that are powered by state-of-the-art gas chromatography coupled to mass spectrometry (GC-MS/MS) or UPLC-MS/MS, contributing to elucidating the functions, biological synthesis, metabolism, and networks of phytohormones.

References

  1. Ye T, Hao Y H, Yu L, et al. A simple, rapid method for determination of melatonin in plant tissues by UPLC coupled with high resolution orbitrap mass spectrometry. Frontiers in plant science, 2017, 8: 64.
  2. Erland L A E, Chattopadhyay A, Jones A M P, et al. Melatonin in plants and plant culture systems: Variability, stability and efficient quantification. Frontiers in plant science, 2016, 7: 1721.
  3. Arnao M B, Hernández‐Ruiz J. Melatonin as a regulatory hub of plant hormone levels and action in stress situations. Plant Biology, 2020.
For Research Use Only. Not for use in diagnostic procedures.

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