Baicalein Analysis Service

Baicalein is one of the most abundant flavonoids in Scutellaria baicalensis. Baicalein is the active ingredient in the body. Baicalein is rapidly converted into baicalin and other metabolites in the blood after entering the animal body. It has numerous pharmacological effects and has been widely studied in the field of biomedicine.

Baicalein has strong antibacterial and antiviral effects. The effect of baicalin on viral diseases may be related to its ability to inhibit viral replication and protect host cells. Studies have shown that baicalin is able to resist human cytomegalovirus (HCMV) and to prevent and treat AIDS. Baicalin has been investigated for the treatment of diseases related to free radicals and oxidative stress because of its oxygen radical scavenging and antioxidant effects. It can also inhibit the proliferation of various tumor cells and has significant anti-tumor activity. It can also reduce cerebral vascular resistance, improve cerebral blood circulation, increase cerebral blood flow, and have anti-platelet coagulation effects. In addition, it also plays a different degree of sway in liver, nerve, antipyretic and anti-inflammatory.

Due to the wide range of applications for baicalein, it is important to establish analytical methods for baicalein. Creative Proteomics can provide you with analytical services for baicalein content based on high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) technology to accelerate the progress of your project.

Applications of Baicalein Analysis

• Qualitative analysis of the main types of baicalein
• Quantitative analysis of baicalein content
• Ensure safety and quality
• Provide basis for improvement

Advantages of Our Baicalein Analysis Service

• Strong technical and management team.
• Professional integration of the service platform.
• Database of baicalein analysis methods.
• Continuous technological innovation.

Service Workflow

Our newly developed sample preprocessing method provides safety, environmental protection, rapidness, effectiveness, and the average recovery rate is over 90%. Sample preprocessing at Creative Proteomics complies with the in-house standard operation procedures (SOP), ensuring maximized homogeneity between samples.

Figure 1. Baicalein analysis service workflow.

Monitoring mode: ESI-MS positive ion monitoring mode

Mobile phase: formic acid water - formic acid methanol

Linear range: r>0.99

Elution time: dozens of minutes, slightly different according to different ingredients

Analysis content:

• Standard curve creation
• Raw data preprocessing
• Absolute quantitation of baicalein
• Differential metabolites screening
• Optimal analyses

Sample Requirements

• Plant tissues: 200mg/sample
• Animal tissues: 200mg/sample
• Blood/plasma: 500 ul/sample
• Urine: 500 ul/sample
• Cells: 1x10⁷/sample

We can also accept other types of samples. Please contact our technical staff for specific testing requirements.

Delivery

• Experimental procedure
• Parameters of HPLC-MS/MS
• Purity analysis report
• HPLC-MS/MS raw data files and HPLC-MS/MS data quality checks
• Metabolites quantification data
• Custom analysis report

HPLC-MS/MS based profiling of baicalein enables quantitative analyses in a faster, more convenient, and more sensitive manner. With decades of experience in HPLC-MS/MS services, Creative Proteomics has a proven track record supporting diverse baicalein detection and quantitation. We can meet your specific project requirements, from sample to bioinformatics.