Vitamin A Analysis Service

Overview

Vitamin A, also known as retinol, has the functions of promoting the growth of the body and maintaining the integrity of the epidermis. It is a fat-soluble vitamin necessary for the body to maintain normal metabolism and function. Vitamin A, as a micronutrient necessary for growth and metabolism, is mainly used to improve vision and enhance immune system function. It plays an important role in enhancing the body's immunity, and is widely used in nutrient supplements. Mass spectrometry technology has become the gold standard method for the analysis of small molecules in biological samples due to its advantages such as rapidity, high resolution, high sensitivity, and high specificity. It is widely used in clinical research and diagnosis, and is also used to reduce the amount of sample collection and simultaneous detection with one injection. Multivitamins provide a highly effective method. Among them, liquid chromatography mass spectrometry has the characteristics of high sensitivity, high precision, and high throughput. It can detect the low content of vitamin A in samples. A method based on liquid chromatography mass spectrometry to determine vitamin A in the body has been developed. It is becoming more and more important for clinical application.

Creative Proteomics can provide a highly sensitive and rapid high-performance liquid phase tandem mass spectrometry (LC-MS/MS) for the determination of Vitamin A content, which can meet the rapid and high-throughput detection of vitamin A in multiple sample types. The method has high throughput and high accuracy, while sample processing is simple, time-consuming, and has high recovery and accuracy.

Figure 1. Chemical structures of vitamin A main derivatives. (Bar-El 2017).

Applications of Vitamin A Analysis

• Analysis and application of the determination of Vitamin A content
• The quality and safety of nutrient supplements
• Nutritional research and development of medicinal value
• Improve the understanding of the molecular mechanism of vitamin A

Advantages of Our Vitamin A Analysis Service

Highly accurate detection of vitamin A content by LC-MS/MS

Meet the needs of low-content sample testing and high-throughput testing

Provide multi-vitamin type detection technology

Fast turnaround time and high-quality analysis report

One-stop service and low prices

Service Workflow

The LC-MS technology combines the high-efficiency separation capabilities of high performance liquid chromatography with the high resolution and high sensitivity of mass spectrometry. The samples to be tested can be analyzed on the computer without derivatization, which can shorten the analysis time and improve the sensitivity and sensitivity of the analysis. accuracy.

Figure 2. Vitamin A analysis service workflow.

Detection method: standard curve method, internal standard method for quantification

Reaction detection method: electrospray positive and negative ion mode

Precision: 0.00%-15%

Standard recovery rate: 85%-115%

The lowest detection limit: 0.0005-21.42μg/l

Reaction mode: ion monitoring mode

Analysis content:

• Construction of standard curve
• Selection of chromatographic and mass spectrometry conditions
• Screening of sample pretreatment conditions
• Investigation of the calibration range and detection limit
• Precision and accuracy inspection
• Limit of quantification analysis
• Chromatogram raw image and data collection
• Determination of Vitamin A and Its Derivatives

Sample Preparation

• Serum/plasma: 50ul/sample
• Urine: 50µl/sample
• Bile: 10ul/sample
• Tissue: 50mg/sample
• Fresh stool: 50mg/sample
• Freeze-dried stool: 5mg/sample
• Cell: 4×10⁶/sample
• Sample storage and transportation: Store in liquid nitrogen or -80°C, and transport on dry ice.

Number of biological replicates: At least 6 biological replicates for plant samples, and at least 10 biological replicates for animal samples.

Delivery

• Comprehensive experimental procedure
• Sample pretreatment and purification analysis
• Mass spectrum raw image and data analysis report
• Determination of Vitamin A and its derivatives
• Customized analysis report

The LC-MS/MS can perform high-sensitivity and high-selectivity determination of the vitamin A content of low-content samples, and the analysis cycle is short, which can meet the requirements of high-throughput detection of vitamin A. Creative Proteomics has rich experience and good reputation in the detection of vitamin content information, we are wholeheartedly committed to providing you with quality services.

References

1. Bar-El Dadon S, Reifen R. Vitamin A and the epigenome. Critical Reviews in Food Science and Nutrition. 2017;57(11):2404-2411.
2. Chatelaine H, Dey P, Mo X, et al. Vitamin A and D Absorption in Adults with Metabolic Syndrome versus Healthy Controls: A Pilot Study Utilizing Targeted and Untargeted LC-MS Lipidomics. Molecular Nutrition & Food Research. 2021;65(2):e2000413.