Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Cinene Analysis Service

Cinene Analysis Service

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Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

About cinene

Cinene, also known as limonene, is a monoterpene compound.

Its molecular formula is C10H16, and its CAS number is 138-86-3.

It is also a natural functional monoterpene. It is widely used as a flavor and fragrance additive in food. It is a colorless oily liquid with a lemon-like fragrance.

cinene has good antitussive, expectorant, and antibacterial effects. Compound cinene can be used clinically to promote choleretics, dissolve stones, promote the secretion of digestive juice and eliminate intestinal gas.

The molecular structure of cineneThe molecular structure of cinene

The technical route of targeted metabolomics of cinene

The technical route of targeted metabolomics of cinene

Sample requirements

Sample requirements
Sample type
Cell sample - please centrifuge after sampling, remove the medium, immediately put it in liquid nitrogen, and store it at -80°.
     Tissue sample - please put it in liquid nitrogen immediately after separation and store it at -80°.
Sample mixed
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
     ※ The same condition refers to the same period, basically the same phenotype, and the same part.
Sample weight and repeat
Samples that have been lyophilized are >0.5g.
     For samples with low water content, such as stems, flowers, seeds, dormant buds, etc., fresh samples >1g.
     For samples with middle water content, roots and leaves (especially young leaves), fresh samples >2g.
     For samples with water content >90%, if fruit (tomato, watermelon, citrus, etc.), fresh sample >5g.
     It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature.
     Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation).
     There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

How to order

Order online

   In the online consultation below or dial the consultation phone, we will arrange staff to contact you as soon as possible.

Mail order

   Send detailed consultation information to the mailbox, we will arrange staff to reply to you as soon as possible, and contact you.

Creative Proteomics offers several approaches to plant metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Saint-Lary L, Roy, Céline, Paris J P, et al. Metabolomics for the authentication of natural extracts used in flavors and fragrances: the case study of violet leaf absolutes from viola odorata[J]. Chemistry & Biodiversity, 2016, 13(6):737-747.
  2. Mohammed A, Hussain R M, Ur R N, et al. De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.) [J]. DNA Research (6):6.
  3. Shimizu T, Watanabe M, Fernie A R, et al. Targeted LC-MS analysis for plant secondary metabolites[M]. Plant Metabolomics. 2018.
For Research Use Only. Not for use in diagnostic procedures.

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