Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Phytol Analysis Service

Phytol Analysis Service

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Phytol, a branched chain on the chlorophyll molecule, is an acyclic diterpene alcohol consisting of four isoprene units and a lipophilic aliphatic chain that determines the lipid solubility of chlorophyll. It can be used as a precursor for the manufacture of synthetic forms of vitamin E and vitamin K1. Plant material ingested by ruminants will release phytol through intestinal fermentation. Based on LC-MS technology, Creative Proteomics provides qualitative and quantitative analysis of phytol.

The molecular structure of phytol The molecular structure of phytol

The technical route of targeted metabolomics of phytol

The technical route of targeted metabolomics of phytol

Sample requirements

Sample requirements
Sample type
Cell sample - please centrifuge after sampling, remove the medium, immediately put it in liquid nitrogen, and store it at -80℃.
Tissue sample - please put it in liquid nitrogen immediately after separation and store it at -80℃.
Sample mixed
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
※ The same condition refers to the same period, basically the same phenotype, and the same part.
Sample weight and repeat
Samples that have been lyophilized are >0.5g.
For samples with low water content, such as stems, flowers, seeds, dormant buds, etc., fresh samples >1g.
For samples with middle water content, roots and leaves (especially young leaves), fresh samples >2g.
For samples with water content >90%, if fruit (tomato, watermelon, citrus, etc.), fresh sample >5g.
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature.
Dry ice transportation (about 3~4kg dry ice is consumed every day, please use sufficient dry ice for transportation).
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.

How to order

Order online

In the online consultation below or dial the consultation phone, we will arrange staff to contact you as soon as possible.

Mail order

Send detailed consultation information to the mailbox, we will arrange staff to reply to you as soon as possible, and contact you.

Creative Proteomics offers several approaches to plant metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Gachet, MS, Schubert, et al. Targeted metabolomics shows plasticity in the evolution of signaling lipids and uncovers old and new endocannabinoids in the plant kingdom[J]. SCI REP-UK, 2017, 2017,7
  2. Mohammed A, Hussain R M, Ur R N, et al. De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.) [J]. DNA Research (6):6.
  3. Shimizu T, Watanabe M, Fernie A R, et al. Targeted LC-MS analysis for plant secondary metabolites[M]. Plant Metabolomics. 2018.
For Research Use Only. Not for use in diagnostic procedures.

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