Metabolomics Creative Proteomics

Phytol Analysis Service

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What is Phytol?

Phytol, chemically known as 3,7,11,15-tetramethyl-2-hexadecen-1-ol, is a long-chain alcohol closely associated with chlorophyll, the pigment responsible for capturing light energy during photosynthesis. It can be found in the lipid tail of the chlorophyll molecule and is released during the breakdown of chlorophyll.

The molecular structure of phytolThe molecular structure of phytol

Creative Proteomics offers a comprehensive range of services for the qualitative and quantitative analysis of phytol. Qualitative analysis focuses on identifying the presence of phytol in a given sample. This can be crucial in research, where the confirmation of phytol's presence is a primary goal. It is also a prerequisite for quantitative analysis. Quantitative analysis aims to precisely measure the concentration of phytol in a sample. Accurate quantification is necessary in various fields, including pharmaceuticals, agriculture, and food science, where the exact amount of phytol can influence the product's quality and effectiveness.

What We Offer

Qualitative Analysis

Our qualitative analysis services are the gateway to confirming the presence of phytol in your samples. This initial step is essential for researchers looking to validate hypotheses and explore the occurrence of phytol in various matrices. With our rigorous qualitative approach, we can unequivocally identify the presence of phytol in your samples.

Quantitative Analysis

For industries and research projects where precision matters, our quantitative analysis services step in. We go beyond mere identification and provide you with precise measurements of phytol concentrations in your samples. This data is vital for quality control, formulation adjustments, and scientific investigations where the exact quantity of phytol plays a pivotal role.

Workflow for phytol analysisWorkflow for phytol analysis

Diverse Sample Types

Creative Proteomics recognizes that phytol analysis is needed across a spectrum of sample types. Our services encompass a wide range, including:

  • Plant Extracts: Covering various plant species.
  • Algae Samples: From freshwater to marine algae species.
  • Soil Samples: Extracted from phytol-rich environments.
  • Dietary Supplements: Evaluating phytol content in supplements.
  • Cosmetics: Analyzing products containing phytol.
  • Biofuels: Ensuring biodiesel and other fuels meet phytol specifications.
  • Pharmaceutical Samples: Testing pharmaceuticals with phytol content.
  • Drug Intermediates: Assessing intermediate compounds in drug synthesis.

Our expertise transcends boundaries, enabling us to provide phytol analysis services for a multitude of applications.

Advanced Analytical Techniques

To deliver precise and dependable results, we employ state-of-the-art analytical techniques, primarily based on mass spectrometry:

  • Gas Chromatography-Mass Spectrometry (GC-MS): Our GC-MS analysis combines gas chromatography with mass spectrometry, utilizing the highly sensitive Agilent 7890A GC System coupled with the Agilent 5975C MSD. This technique ensures precision and resolution in the analysis of Phytol across diverse samples.
  • Liquid Chromatography-Mass Spectrometry (LC-MS): Our LC-MS analysis utilizes the Thermo Fisher Q Exactive series, a powerful tool for the analysis of complex samples. This technique provides accurate results, even in the presence of challenging matrices.

Customized Solutions

We understand that no two projects are the same. That's why we offer customization options for our phytol analysis services. We work closely with our clients to tailor our services to their unique requirements, ensuring that the data we provide is directly relevant to their objectives.

Sample Requirements for Phytol Analysis

Sample TypeSample VolumeStorage TemperaturePreparation Requirements
Plant Extracts1-2 mL-20°CFreeze samples immediately after collection.
Algae Samples5-10 g-80°CFreeze samples promptly.
Soil Samples50-100 g-20°CEnsure samples are well-sealed to prevent contamination.
Dietary Supplements2-5 gRoom temperatureKeep samples in original packaging.
Cosmetics1-2 gRoom temperatureProvide samples in their original containers.
Biofuels1-2 mL-20°CStore samples in airtight containers.
Medicinal Products1-2 gRoom temperatureAvoid exposure to light and moisture.
Drug Intermediates1-2 gRoom temperatureSubmit samples in well-sealed containers.
Food Items2-5 g-20°CKeep samples in airtight packaging.
Water Samples50-100 mL4°C (refrigerated)Collect samples in clean, sterile containers.
Crop Samples10-20 g-20°CFreeze samples promptly after collection.
Veterinary Medications1-2 gRoom temperatureEnsure samples are tightly sealed and labeled.

Case: Chemical Composition and Antioxidant Activities of Essential Oils from Celtis zenkeri Engl.


Essential oils (EOs) are volatile compounds derived from plant metabolism and have garnered attention for their potential medicinal applications. Celtis zenkeri Engl., a plant from the Cannabaceae family, is traditionally used in Nigeria for various therapeutic purposes. This study aims to analyze the chemical composition and antioxidant properties of EOs extracted from the leaves and stem bark of Celtis zenkeri Engl., a previously unreported research endeavor.


The leaves and stem bark of Celtis zenkeri Engl. were collected in Nigeria, specifically in Ikire, Osun State. The plant was identified and authenticated, with voucher specimens deposited at the Forest Research Institute of Nigeria.

Technique Methods

Extraction of Essential Oils: The EOs were obtained from the plant material via hydrodistillation using a British Pharmacopoeia-compliant distillation unit.

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis: The chemical composition of the EOs was analyzed using GC-MS.

Antioxidant Assay: The antioxidant activity of the EOs was assessed by their ability to scavenge the DPPH+ radical using spectrophotometric measurements.


  • A total of 23 compounds were identified in the leaf EO, representing 99.9% composition, while nine compounds were found in the stem bark EO, accounting for 98.9% composition.
  • Phytol was the major compound in both EOs, with the leaf EO containing 46.1% and the stem bark EO containing 27.0%.
  • The EOs exhibited good radical scavenging activities due to the presence of chemical constituents, including phytol and α-caryophyllene.
  • The antioxidant activity of the leaf EO was comparable to that of standard antioxidants like ascorbic acid and butylated hydroxyl anisole (BHA).
  • The stem bark EO also displayed strong antioxidant activity and outperformed α-tocopherol, a known antioxidant.

The GC-MS chromatogram of the leaf of C. zenkeri Engl. essential oil.The GC-MS chromatogram of the leaf of C. zenkeri Engl. essential oil.

The GC-MS chromatogram of the stem bark of C. zenkeri Engl. essential oil.The GC-MS chromatogram of the stem bark of C. zenkeri Engl. essential oil.


  1. Okpala, Ejike Onwudiegwu, Patricia Akpomedaye Onocha, and Muhammad Shaiq Ali. "Antioxidant activity of phytol dominated stem bark and leaf essential oils of Celtis zenkeri Engl." Trends in Phytochemical Research 6.2 (2022): 137-144.
For Research Use Only. Not for use in diagnostic procedures.


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