Elaidic Acid Analysis Service

Elaidic acid (9-octadecenoic acid, trans-oleic acid) is a trans fatty acid commonly found in hydrogenated vegetable oils. The dangers of trans fatty acids are becoming more and more well known.

The formation mechanism of trans fatty acids also varies. The trans fatty acids in food mainly come from the fatty acids with trans structures formed during the metabolism of ruminants and during food processing. The trans fatty acids in ruminants are formed by the enzymatic hydrogenation of the hydrogenating bacteria-Vibrio butyricum. In the hydrogenation process of vegetable oil, the trans isomer is produced in the presence of metal catalysts. Under high cooking conditions (150° or higher) oleic acid isomerizes to form elaidic acid.

TFA increases total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) levels and decreases HDL-C levels. 9t-C18:1 has also been shown to induce apoptosis in endothelial cells and human hepatocellular carcinoma cells.

In order to better promote research on disease mechanisms, master the content of trans-oleic acid in various foods and products, and accelerate research in the direction of human health, Creative Proteomics has established a liquid chromatography-mass spectrometry (LC-MS) detection platform for the qualitative and quantitative determination of elaidic acid. In addition, we offer other fatty acid assays to meet your different needs.

Service Workflow

Sample Requirements

We analyze a wide range of biological materials and can also accept industrial samples.

• Serum / plasma: ≥ 100 ul/sample
• Tissues: ≥ 100 mg/sample
• Foods dry weight: ≥ 200 mg
• Drugs
• Others

Want to analyze other samples? Please contact our technical staff.

Deliverables

• Experimental protocols
• Instrument parameters
• MS raw data files and MS data quality checks
• Absolute quantitative data of elaidic acid
• Bioinformatics analysis report

If you want to test other fatty acids or have other questions, please contact us and we will be happy to answer them for you. We look forward to working with you.