Arachidonic Acid Metabolism Service

Overview

Arachidonic acid is a 20-carbon polyunsaturated fatty acid and the central eicosanoid precursor in mammalian cells.  It is structurally related to the saturated arachidic acid found in cupuaçu butter. Since arachidonic acid cannot be synthesized de novo from animal cells, most of arachidonic acid in the animal is derived from linoleic acid, which can be obtained only from dietary sources. After biosynthesis of arachidonic acid from its precursor, it is esterified into the phospholipids of the outer cell membranes.  Therefore, the first step in the arachidonic acid metabolism is cleavage and release of arachidonic acid from the phospholipid-bound form via phospholipase A2 (PLA2), phospholipase C and phospholipase D (Figure 1). The majority of arachidonic acid metabolites can act both as pro- and anti-inflammatory mediators, modulating gene expression, cytokine signalling and other immune regulatory factors. So the aberrant arachidonic acid metabolism is closely related to a variety of human diseases like gastrointestinal ulceration and cancer. In Creative Proteomics, we provide reliable, rapid and cost-effective arachidonic acid metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

Figure 1. The classical arachidonic acid metabolism pathway.

The Main Applications of Arachidonic Acid Metabolism Service

Identifying novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Our Arachidonic Acid Metabolism Service

Constantly optimized protocol and analytical software

Professional experiment design

Quick turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides arachidonic acid metabolism analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 2). Our service will be tailored to specific samples and needs for optimal results.

Figure 2. The overall workflow of arachidonic acid metabolism.

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of arachidonic acid metabolism, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored arachidonic acid metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Farooqui AA, Horrocks LA. Signaling and interplay mediated by phospholipase A2, C, and D in LA-N-1 cell nuclei. Reprod Nutr Develop. 2005.45:613-631.
2. Adler David H, Cogan Joy D, Phillips John A, et al. Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction. J Clin Invest. 2008.118:2121-2131.