Sphingolipids are a class of lipids with eighteen carbon amino-alcohol backbones synthesized from nonsphingolipid precursors. The different modifications of this basic structure leading to a large number of sphingolipids. Although sphingolipids contain various structures and functions, the biosynthesis and degradation pathway for these sphingolipids are the same. Therefore, the sphingolipid metabolism is a mutual network different from a single common biosynthesis pathway and converge into a single common degradation pathway. The simplest forms of sphingolipids are sphingosine, phytosphingosine and dihydrosphingosine. They function as the backbones for other complex groups of sphingolipids. These compounds play important roles in signal transduction and cell recognition. Disorders of sphingolipid metabolism links to various human diseases like Gaucher disease and Fabry disease. Understanding how the sphingolipid metabolism is regulated via contributes to exploring the underlying mechanism behind human diseases.
Creative Proteomics has established LC-MS/MS analysis technology that can provide you with reliable, rapid and cost-effective sphingolipid analysis services to accelerate research goals from disease diagnosis to new insights into disease mechanisms or treatments.
Advantages of Our Sphingolipid Metabolism Service
Constantly optimized protocol and analytical software
Professional experiment design
Quick turnaround time
High accuracy, specificity, and sensitivity
List of Partial Detectable Sphingolipid Related Metabolites at Creative Proteomics
|Sphingoid bases||Sphingosine, Dihydrosphingosine, Phytosphingosine, Sphingosyl-phosphocholine, Sphinganine 1-phosphate|
|Ceramides||Phytoceramide, Dihydroceramide, Digalactosylceramide, Galactosylceramide, Glucosylceramide, Lactosylceramide|
|Inositol-containing ceramides||Inositol phosphorylceramide, mannose inositol phosphorylceramide, and mannose diinositol phosphorylceramide.|
|Glycosphingolipids||Cerebrosides, Sulfatides, Gangliosides|
We can analyze a wide range of biological materials including but not limited to cells and solid tissues. If you need transport your samples to us, please follow the following requirements for different types of samples:
- Blood/plasma: 500ul/sample
- Urine: 1ml/sample
- Tissue: 200mg/sample
- Cells: 1x107/sample
- Feces: 500mg/sample
Shipment condition: dry ice
- Experimental procedures
- The parameters of liquid chromatograph and mass spectrometer
- MS raw data files and MS data quality inspection
- Absolute quantitative analysis data
Based on advanced LC-MS/MS platforms for the determination of sphingolipid metabolism, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored sphingolipid metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research. If you would like additional information or need to analyse other compounds, please contact us.
- Kolter Thomas, Sandhoff Konrad. Sphingolipid metabolism diseases. Biochim Biophys Acta. 2006.1758:2057-2079.
- Hannun Yusuf A, Obeid Lina M. Sphingolipids and their metabolism in physiology and disease. Nat Rev Mol Cell Biol. 2018.19:175-191.
For Research Use Only. Not for use in diagnostic procedures.