Fatty Acid Biosynthesis Service

Fatty acid synthesis is the generation of fatty acids from acetyl-CoA through the action of enzymes called fatty acid synthases. This process takes place in the cytoplasm. Most of the acetyl-CoA for synthesizing fatty acids is from carbohydrates via the glycolytic pathway. The glycolytic pathway also provides the glycerol with which three fatty acids can combine to form triglycerides. When only two fatty acids combine with glycerol and the third alcohol group is phosphorylated with a group like phosphatidylcholine, a phospholipid is formed. Phospholipids form the lipid bilayers which consist of cell membranes and surround the organelles within the cells. It would not be surprising that fatty acid synthesis plays an important role in maintaining the function of cell and organelles. This cycle of eight different enzymes.  Its abnormal among these enzymes closely links to various human diseases like combined malonic and methylmalonic aciduria and leukodystrophy. In Creative Proteomics, we provide reliable, rapid and cost-effective fatty acid synthesis related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Fatty Acid Biosynthesis Service

Identifying novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Our Fatty Acid Biosynthesis Service

Constantly optimized protocol and analytical software

Professional experiment design

Quick turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides fatty acid biosynthesis analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of fatty acid biosynthesis.

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of fatty acid biosynthesis, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored fatty acid biosynthesis analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Sloan Jennifer L, Johnston Jennifer J, Manoli Irini, et al. Exome sequencing identifies ACSF3 as a cause of combined malonic and methylmalonic aciduria. Nat Genet. 2011.43:883-886.
2. Lamari F, Mochel F, Sedel F, et al. Disorders of phospholipids, sphingolipids and fatty acids biosynthesis: toward a new category of inherited metabolic diseases. J Inherit Metab Dis. 2013. 36:411-425.