Unsaturated Fatty Acids Analysis Service

What are Unsaturated Fatty Acids?

Unsaturated fatty acids refer to the fatty acids with one or more double/triple carbon-carbon bonds in the carbon chain. Basically, they can be divided into three classes: monounsaturated fatty acids, polyunsaturated fatty acids and acetylenic fatty acids. Unsaturated fatty acids occurs through sequential reactions of alternating desaturation and two-carbon chain elongation of the main precursors: stearic (18:0), linoleic (18:2) and linolenic acid (18:3) of the n-9, n-6 and n-3 series, respectively, of polyunsaturated fatty acids (PUFA). The sequential procedure of these reactions is also called desaturation and requires a variety of desaturases. As one of important nutrition in animals, unsaturated fatty acids have crucial roles in maintaining animal health. Therefore, the aberrant unsaturated fatty acids pathway causes several pathologies like diabetes and cancer.

At Creative Proteomics, we are committed to providing high-quality and customized unsaturated fatty acid analysis solutions to meet your research needs. Our team of experienced scientists uses state-of-the-art equipment and cutting-edge techniques to provide accurate and reliable results. We also offer personalized support throughout the analysis process, from sample preparation to data analysis.

Applications of Unsaturated Fatty Acid Analysis Services

• Nutrition: Helps assess the fatty acid composition of foods and assess their health benefits. For example, analyze fish oil supplements for UFA content to ensure they contain adequate amounts of EPA and DHA.
• Metabolism: Helping identify disease biomarkers and understand underlying mechanisms of disease. Examples include analyzing UFA levels in blood or tissue samples to identify changes in fatty acid metabolism that may contribute to the development of metabolic diseases.
• Drug development: Assessing the effect of drugs on fatty acid metabolism. For example, analyzing the UFA content of cells or tissues treated with drugs to identify changes in the fatty acid profile that may contribute to the drug's therapeutic effect.

List of Detectable Unsaturated Fatty Acids at Creative Proteomics

Service Workflow

Creative Proteomics provides unsaturated fatty acids analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of unsaturated fatty acids

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from animals. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
  Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of unsaturated fatty acids, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored unsaturated fatty acids analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Lee Hyungjae, Park Woo Jung. Unsaturated fatty acids, desaturases, and human health. J Med Food. 2014.17:189-197.
2. Tamura Kenji, Makino Asami, Hullin-Matsuda Françoise, et al. Novel lipogenic enzyme ELOVL7 is involved in prostate cancer growth through saturated long-chain fatty acid metabolism. Cancer Res. 2009. 69:8133-8140.