Fatty Acid Elongation Service

Overview

Longer chain fatty acids is very important for cells. Although longer chain fatty acids are derived from either dietary sources or from elongation of C16-CoA or C18-CoA formed by the cytoplasmic fatty acid synthetase system, all of the fatty acids needed by the body can be synthesized from palmitate (C16:0) except the essential, polyunsaturated fatty acids like linoleate. Elongation of fatty acids could happen at endoplasmic reticulum in which fatty acid molecules of length up to C24 may be produced. Mitochondrial elongation could lead to fatty acids up to C16 in length. Fatty acid elongation in mitochondria is essentially the reverse of beta-oxidation for fatty acid oxidation. Particularly, both pathways make use of acetyl-CoA acyltransferase, 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase. Any step in these pathways will impair the function of cell. A series of studies have shown that abnormal fatty acid elongation causes several pathologies like neuronal ceroid lipofuscinosis and fundus flavimaculatus. In Creative Proteomics, we provide reliable, rapid and cost-effective fatty acid synthesis related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Fatty Acid Elongation Service

Identifying new biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Our Fatty Acid Elongation Service

Constantly optimized protocol and analytical software

Professional experiment design

Quick turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides fatty acid elongation analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of fatty acid elongation

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the below requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of fatty acid elongation, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored fatty acid elongation analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Smith Katherine R, Dahl Hans-Henrik M, Canafoglia Laura, et al. Cathepsin F mutations cause Type B Kufs disease, an adult-onset neuronal ceroid lipofuscinosis. Hum Mol Genet. 2013. 22:1417-1423.
2. Michaelides Michel, Gaillard Marie-Claire, Escher Pascal, et al. The PROM1 mutation p.R373C causes an autosomal dominant bull's eye maculopathy associated with rod, rod-cone, and macular dystrophy. Invest Ophthalmol Vis Sci. 2010.51:4771-4780.