Glycerophospholipid Metabolism Service

Overview

Glycerophospholipids are glycerol-based phospholipids containing at least one O-1-acyl, O-1-alkyl, or O-1-alkenyl residue attached to the glycerol moiety. The presence of an additional head group (such as choline, serine and glycerol) attached to the phosphate diversifies glycerophospholipids (Figure 1), glycerophospholipids present a significant challenge in high level of accuracy in lipid quantitation. Both biosynthesis (CDP-DAG pathway and Kennedy pathway) and degradation (different phospholipases) of glycerophospholipids are controlled by different signalling pathways. therefore, the metabolism of glycerophospholipids is pretty complicated and it is not fully understood how and when their substitutions and modifications occur. But it is quite documented that aberrant glycerophospholipids metabolisms are closely associated with various human pathologies like Laurence-Moon syndrome and cancer. Understanding the glycerophospholipid metabolisms contribute to unravelling the underlying mechanism behind human diseases. In Creative Proteomics, we provide reliable, rapid and cost-effective glycerophospholipid metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

Figure 1. The overview of glycerophospholipid metabolism.

The Main Applications of Glycerophospholipid Metabolism Service

Identifying novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Glycerophospholipid Metabolism Service

Constantly optimized protocol and analytical software

Professional experiment design

Quick turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides glycerophospholipid metabolism analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 2. The overall workflow of glycerophospholipid metabolism.

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of fatty acid biosynthesis, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored lipid metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Hufnagel Robert B, Arno Gavin, Hein Nichole D, et al. Neuropathy target esterase impairments cause Oliver-McFarlane and Laurence-Moon syndromes. J Med Genet. 2015.52:85-94.
2. Huang Chunfa, Freter Carl. Lipid metabolism, apoptosis and cancer therapy. Int J Mol Sci. 2015.16:924-949.