Ether Lipid Metabolism Service

Overview

Ether lipids are a specific class of glycerophospholipids that own an alkyl chain attached to the sn-1 position via an ether bond. Ether lipids consist of about one-fifth of the total phospholipid pool, but tissue distribution varies. The highest levels are found in the brain, heart, spleen, and white blood cells, while liver has subtle amount of intracellular ether lipid. Ether lipids are synthesized in a common pathway that begins with the association of the peroxisomal matrix enzymes on the luminal side of the peroxisome membrane and completes in the endoplasmic reticulum. It is well documented that that ether lipids are involved in a variety of biological functions including regulating cell differentiation, impacting cellular signalling, and reducing oxidative stress through their ability to function as potential endogenous antioxidants. Emerging studies suggest that altered ether lipid production is associated with several disorders including neurodegenerative diseases, cancer and metabolic disorders. Understanding the ether lipid metabolism contributes to exploring the underlying mechanism behind human diseases. In Creative Proteomics, we provide reliable, rapid and cost-effective ether lipid metabolism related service to speed up your research aims ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Ether Lipid Metabolism Service

Identifying novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Our Ether Lipid Metabolism Service

Constantly optimized protocol and analytical software

Professional experiment design

Quick turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides ether lipid metabolism analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four main procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis (Figure 1). Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of ether lipid metabolism.

List of Partial Detectable Ether Lipid Metabolism Related Metabolites at Creative Proteomics

2-Acetyl-1-alkyl-sn-glycero-3-phosphate	1-Alkyl-sn-glycero-3-phosphocholine	1-Alkyl-2-acylglycerol	Platelet-activating factor
2-Acyl-1-alkyl-sn-glycero-3-phosphocholine	1-Alkyl-sn-glycero-3-phosphate	Plasmenylethanolamine	Plasmanylethanolamine
2-Acyl-1-(1-alkenyl)-sn-glycero-3-phosphate	Galactosyl-1-alkyl-2-acylglycerol	1-Alkyl-2-acetyl-sn-glycerol	Seminolipid

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of ether lipid metabolism, professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored ether lipid metabolism analysis service with rapid experimental procedures and easy to read report, to accelerate your scientific research.

References

1. Braverman NE, Moser AB. Functions of plasmalogen lipids in health and disease. Biochim Biophys Acta. 2012.1822:1442-1452.
2. Dean John M, Lodhi Irfan J. Structural and functional roles of ether lipids. Protein Cell. 2018.9:196-206.