Metabolomics Creative Proteomics

Ketone Bodies Metabolism Analysis Service

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What are Ketone Bodies?

Ketone bodies, also known as ketones, refer to three specific molecules: acetoacetate, beta-hydroxybutyrate, and acetone. They are produced in the liver from fatty acids during times of reduced glucose availability, such as fasting, prolonged exercise, or a low-carbohydrate diet. Ketone bodies are an alternative energy source for the body, particularly the brain and muscles, when glucose levels are insufficient.

Comprehensive Ketone Bodies Metabolism Analysis Services by Creative Proteomics

At Creative Proteomics, we offer a wide-ranging and comprehensive suite of services dedicated to the analysis of ketone bodies and their metabolism. Our services are designed to provide in-depth insights into the quantitative analysis of ketone bodies, related metabolites, and their roles in various physiological and pathological conditions.

1. Quantitative Analysis of Ketone Bodies: We utilize highly sensitive mass spectrometry techniques, including Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS), to precisely quantify the three major ketone bodies: acetoacetate, beta-hydroxybutyrate, and acetone. These quantitative data are invaluable for research and diagnostic purposes.

2. Analysis of Related Metabolites: In addition to ketone bodies, we can analyze other metabolites closely associated with ketone bodies metabolism, such as acetyl-CoA, acetoacetyl-CoA, succinyl-CoA, and more. This provides a comprehensive view of metabolic pathways.

3. Quantitative Analysis of Metabolic Pathways: Our services encompass metabolic pathway analysis, allowing us to determine the roles of ketone bodies in various metabolic pathways, including the TCA cycle, fatty acid metabolism, biosynthesis, and more.

4. Stable Isotope Labeling Studies: We conduct stable isotope labeling experiments to investigate the synthesis, utilization, and exchange of ketone bodies. This sheds light on the roles of ketone bodies in different physiological states.

5. Analysis of Various Biological Sample Types: Our expertise extends to the analysis of ketone bodies in diverse biological sample types, including serum, urine, tissue samples, and even cerebrospinal fluid.

6. Research in Animal and Cell Models: Beyond human samples, Creative Proteomics can extend its analysis services to animal models and cell culture studies, providing insights into ketone bodies metabolism in different biological systems.

7. Pathological Metabolism Research: Our services can be applied to investigate metabolic disorders, such as diabetes and ketoacidosis, aiding in diagnosis and treatment planning.

8. Customized Analysis Solutions: We understand that each research project has unique requirements. Therefore, we offer the flexibility to tailor analysis solutions to meet specific research or diagnostic needs.

List of Ketone Bodies and Related Metabolites Analyzed

CategorySubstances Analyzed
Ketone BodiesAcetoacetate, Beta-Hydroxybutyrate, Acetone, Acetoacetol, 3-Hydroxy-2-butanone, Acetoin, Acetylmethylcarbinol, Acetohexamide, Acetylpyrazine, Acetylacetone, Acetohydroxamic Acid, Alpha-Acetylpyridine, Alpha-Hydroxybutyrate, Alpha-Hydroxyisovalerate, Alpha-Ketobutyrate, Alpha-Ketoglutarate, and more...
Ketone Body IntermediatesAcetyl-CoA, Acetoacetyl-CoA, Isobutyryl-CoA, Propionyl-CoA, Succinyl-CoA, Acetoacetyl-CoA, Malonyl-CoA, Alpha-Ketobutyryl-CoA, Alpha-Ketohexadecenoic Acid, Alpha-Ketomethylvaleric Acid, Beta-Hydroxyisovaleric Acid, Beta-Hydroxyisovaleryl-CoA, Beta-Ketoadipate, Beta-Ketobutyrate, Beta-Ketohexanoic Acid, Beta-Ketohexanoyl-CoA, and more...
Related MetabolitesPyruvate, Lactate, Citrate, Malate, Fumarate, Succinate, Glutamate, Alanine, Aspartate, Glutamine, Leucine, Isoleucine, Valine, and more...

Analytical Techniques for Ketone Bodies Metabolism Analysis

Gas Chromatography-Mass Spectrometry (GC-MS): Agilent 7890B GC-MS System

The Agilent 7890B GC-MS system stands as a pivotal component of our analytical resources. This advanced device seamlessly combines gas chromatography with mass spectrometry to separate and identify ketone bodies. Its distinguishing features include exceptional chromatographic resolution and precise mass measurements, ensuring dependable quantification of acetoacetate, beta-hydroxybutyrate, and acetone in biological specimens.

Liquid Chromatography-Mass Spectrometry (LC-MS): Thermo Fisher Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer

For the high-resolution and sensitive examination of ketone bodies, we rely on the Thermo Fisher Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer. This state-of-the-art apparatus is celebrated for its high-resolution accurate mass (HRAM) capabilities, enabling us to conduct meticulous LC-MS analyses. It excels, particularly, in the discernment and quantification of ketone bodies and their associated metabolites.

Sample Requirements for Ketone Body Metabolism Analysis

Sample TypeSample VolumeStorage ConditionsSpecial Considerations
Serum/Plasma0.5-1 mL-80°C or belowFast sample processing
Urine1-2 mL-80°C or belowMinimize light exposure
Tissue Homogenate20-50 mg tissue-80°C or belowRapid homogenization
Cerebrospinal Fluid0.5-1 mL-80°C or belowMinimize freeze-thaw cycles
For Research Use Only. Not for use in diagnostic procedures.


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