Steroid Biosynthesis Service

Overview

A steroid is an organic compound with four rings arranged in a specific configuration, such as dietary lipid cholesterol, estradiol, testosterone and dexamethasone. Steroid biosynthesis (steroidogenesis) begins with the transfer of free cholesterol from intracellular stores into the inner mitochondrial membrane, where it is transformed into pregnenolone. The conversion of cholesterol to pregnenolone is the rate-limiting step of steroidogenesis and occurs in the single substrate-binding pocket of the cholesterol side-chain cleavage enzyme. Steroids function as important components of cell membranes and many of them are signalling molecules. The malfunction in steroid biosynthesis disrupts the cellular functions and leads to a wide range of human diseases like Lysosomal acid lipase deficiency and Smith-Lemli-Opitz syndrome. In Creative Proteomics, we provide reliable, rapid and cost-effective steroid biosynthesis related analysis service to speed up your research purposes ranging from diseases diagnosis to gaining new insights in disease mechanism or treatment.

The Main Applications of Steroid Biosynthesis Service

Identifying novel biomarkers of diseases

Improving diagnose diseases

Accelerating development of novel drugs

Gaining new mechanism of diseases

Advantages of Our Steroid Biosynthesis Service

Constantly optimized protocol and analytical software

Professional experiment design

Quick turnaround time

High accuracy, specificity, and sensitivity

Service Workflow

Creative Proteomics provides steroid biosynthesis analysis service in a reliable and effective manner, based on our cutting-edge high-performance liquid chromatography (HPLC) platforms. The experimental procedures contain four major procedures: sample collection, metabolites extraction, HPLC data analysis and bioinformatics analysis. Our service will be tailored to specific samples and needs for optimal results.

Figure 1. The overall workflow of steroid biosynthesis.

List of Partial Detectable Steroid Biosynthesis Related Metabolites at Creative Proteomics

Cholesterol	Pregnenolone	17-OH-pregnenolone	Dehydroepiandrosterone	11-deoxycortisol
Progesterone	17-OH-progesterone	Androstenedione	Estrone	Cortisol
Androstanedione	11-deoxycorticosterone	Corticosterone	Aldosterone	Cortisone
Testosterone	Dihydrotestosterone	Estradiol	Estriol

Sample Requirements

We can analyze a wide range of biological materials including but not limited to cells and solid tissues from humans and animals, such as mice, rats, rabbits. If you need transport your samples to us, please follow the following requirements for different types of sample:

• Blood/plasma: 500ul/sample
• Urine: 1ml/sample
• Tissue: 200mg/sample
• Cells: 1x10⁷/sample
• Feces: 500mg/sample
• Shipment condition: dry ice

Report Delivery

• Experimental protocols
• Instrumental factors of HPLC
• The raw data files of HPLC and the summary of HPLC data quality
• Bioinformatics analysis report

Based on advanced HPLC platforms for the determination of steroid biosynthesis professional bioinformatic analysis software and experienced technicians and scientists, Creative Proteomics provides customer-tailored steroid biosynthesis analysis service with rapid experimental procedures and easy to read report, to speed up your scientific research.

References

1. Du H, Sheriff S, Bezerra J, et al. Molecular and enzymatic analyses of lysosomal acid lipase in cholesteryl ester storage disease. Mol Genet Metab. 1998.64:126-134.
2. Correa-Cerro Lina S, Porter Forbes D. 3beta-hydroxysterol Delta7-reductase and the Smith-Lemli-Opitz syndrome. Mol Genet Metab. 2005.84:112-126.