Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, so as to guide your research with reliable and accurate measurement results.
Its IUPAC name is 2-(4-Hydroxyphenyl) chromenylium-3,5,7-triol.
Its molecular formula is C15H11O5.
Its CAS number is 134-04-3.
It mainly found in flowers and berries.
AB Sciex QTRAP® 6500 LC-MS/MS
Three quality control
The molecular structure of pelargonidin
Remove residual impurities in the instrument, provide a stable detection environment, and monitor the stability of the instrument.
Mixed standard mainly monitors the stability of the instrument.
Monitor the stability of the entire extraction process.
Targeted metabolome workflow of pelargonidin
Targeted metabolome studies can establish baseline metabolite levels in tissues or organisms for comparison among experiments under different conditions.
Design experiments and sample processing
Preparation of metabolic standards and LC-MS/MS detection
Make standard curve line
Extraction of metabolites in samples
Raw data collection and preprocessing
Qualitative and quantitative target metabolites
Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)
If it is a cell sample, centrifuge after sampling, remove the medium, immediately put it in liquid nitrogen, and store it at -80°.
If it is a tissue sample, please put it in liquid nitrogen immediately after separation and store it at -80°.
Each sample was mixed from more than 6 tissues under the same conditions and quickly put into liquid nitrogen.
※The same condition refers to the same period, basically the same phenotype, and the same part.
Sample weight and repeat
It is recommended that the total amount of fresh and clean plant samples is >1g, and prepare 6 biological replicates.
Storage and transportation
Dry ice transportation (dry ice volatilizes 3-4 kg /day, please use sufficient dry ice for transportation).
About 1-3 weeks
Creative Proteomics has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of target metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.
- Seo M, Kim H, Lee J H, et al. Pelargonidin ameliorates acetaminophen-induced hepatotoxicity in mice by inhibiting the ROS-induced inflammatory apoptotic response[J]. Biochimie, 2019, 168.
- Samadder A, Tarafdar D, Das R, et al. Efficacy of nanoencapsulated pelargonidin in ameliorating pesticide toxicity in fish and L6 cells:Modulation of oxidative stress and signalling cascade[J]. Science of The Total Environment, 2019, online first(JUN.25):466-473.
- Wang L S, Sun X D, Cao Y, et al. Antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (Raphanus sativus var. niger, Brassicaceae)[J]. Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 2010, 48(10):2712-2718.
For Research Use Only. Not for use in diagnostic procedures.