Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Petunidin Analysis Service

Petunidin Analysis Service

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Petunidin is a natural pigment extracted from the flowers of Pharbitis nil, an annual herb, using modern biotechnology. It can be used in food and beverage coloring, suitable for use under acidic conditions, and also used in cosmetics and as an acid-base indicator.

Petunidin is a promising natural medicine. It has been shown that petunidin can achieve anti-myocardial ischemia-reperfusion injury MIRI by targeting NOX 4, inhibiting ROS production, reducing oxidative stress, regulating Bcl-2 pathway, and preventing myocardial cell apoptosis. Petunidin ameliorates osteopenia by increasing osteoid formation, accelerates osteoblastogenesis, and inhibits bone resorption, which has some osteoprotective effects.

Petunidinchloride is an O-methylated anthocyanin, derived from fleur de sel. Petunidinchloride binds and inhibits the activity of adhesion foci kinase and inhibits platelet-derived growth factor-induced migration of aortic smooth muscle cells, which may have a protective effect against atherosclerosis.

Based on high sensitivity liquid chromatography-mass spectrometry (LC-MS) technology, Creative Proteomics can provide you with qualitative and quantitative analysis of petunidin and petunidin derivatives.

The molecular structure of petunidinThe molecular structure of petunidin

Technical Features

  • Instrument platform: AB Sciex QTRAP® 6500 LC-MS/MS
  • Three quality control
  • Blank: Remove residual impurities in the instrument, provide a stable detection environment, and monitor the stability of the instrument.
  • Mixed standard: Mixed standard mainly monitors the stability of the instrument.
  • Random sample repetition: Monitor the stability of the entire extraction process.

Targeted Metabolomics Analysis of Petuniain

Targeted metabolomic studies can establish baseline metabolite levels in tissues or organisms for comparison among experiments under different conditions.

  • Design experiments and sample processing
  • Standard preparation and mass spectrometry detection
  • Make standard curve
  • Extraction of metabolites in samples
  • Raw data preprocessing
  • Absolute quantification of metabolites based on standard curve
  • Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)

The molecular structure of petunidin

Sample Requirements

  • Tissue ≥ 200 mg/sample
  • Plasma ≥ 200 μL/sample
  • Blood ≥ 200 μL/sample
  • Drugs ≥ 0.2 g/sample

We can also accept other samples. Please contact us for the exact sample size required for the test and our technical staff will communicate with you.

In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample. The same condition refers to the same period, basically the same phenotype, and the same part.

Service Process

Service process

Turnaround time: About 1-4 weeks.

Creative Proteomics has leading technology, first-class R&D and production equipment, and has established a complete metabolome service system. Our professional team has many years of experience in metabolomics, bioinformatics, and statistics, and can help you plan, conduct and report metabolomics research, so that your upstream and downstream experiments can be seamlessly connected. We can provide a wide range of targeted metabolomics services, including target metabolic pathways, target metabolite analysis, etc. You can also perform personalized customization services at the same time during the data analysis stage. If you have any questions or specific requirements, please feel free to contact us.

References

  1. B S E A, B J C A, B P C A, et al. Stability of phenolic compounds, antioxidant activity and colour parameters of a coloured extract obtained from coloured-flesh potatoes - ScienceDirect[J]. LWT, 136.
  2. Duan W , Shao W , Lin W , et al. Integrated metabolomics and transcriptomics reveal the differences in fruit quality of the red and white Fragaria pentaphylla morphs[J]. Food Bioscience, 2021(9):100896.
  3. A L H, A X L, B S L, et al. Transcriptomic and targeted metabolomic analysis identifies genes and metabolites involved in anthocyanin accumulation in tuberous roots of sweetpotato (Ipomoea batatas L.) - ScienceDirect[J]. Plant Physiology and Biochemistry, 2020, 156:323-332.
For Research Use Only. Not for use in diagnostic procedures.

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