Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Pyruvic Acid Analysis Service

Pyruvic Acid Analysis Service

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Pyruvic acid is the simplest alpha-keto acid, with functional groups of carboxylic acids and ketones. Pyruvic acid can be produced from glucose by glycolysis, can be converted back to carbohydrates (e.g. glucose) by gluconeogenesis, or to fatty acids by reaction with acetyl CoA. It can also be used to build the amino acid alanine, converting it to ethanol or lactate by fermentation. When oxygen is present (aerobic respiration), pyruvic acid provides energy to the cell via the citric acid cycle (Krebs cycle), or fermentation in the absence of oxygen to produce lactic acid.

Creative Proteomics' LC-MS-based technology enables the analysis of pyruvate metabolites and metabolic pathways, revealing relevant metabolic mechanisms and validating potential metabolic biomarkers.

Molecular structure of pyruvic acidMolecular structure of pyruvic acid

Technical Route of Targeted Metabolomics of Pyruvic Acid

Technical Route of Targeted Metabolomics of Pyruvic Acid

Sample Requirements

Sample type
Plants, yeasts, microorganisms, etc.
Serum, plasma, urine, bile, bile acid, etc.
Cells, liver, brain tissue and other animal tissues and feces, etc.
There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.
Sample demand
Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
   Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 107, supernatant > 2 ml
   Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
   (Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
Animal tissue / feces related samples > 200mg / sample
For other sample types, please consult technical support or sales.
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
  ※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

  • Experimental steps
  • Related mass spectrometry parameters
  • Part of the mass spectrum picture
  • Raw data

Project Cycle

  • A standard experiment and analysis process takes about 1~4 weeks.

Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Hatoum D, Annaratone C, Hertog M, et al. Targeted metabolomics study of 'Braeburn'apples during long-term storage. Postharvest Biology and Technology, 2014, 96: 33-41.
  2. Griffiths W J, Koal T, Wang Y, et al. Targeted metabolomics for biomarker discovery. Angewandte Chemie International Edition, 2010, 49(32): 5426-5445.
  3. Mohammed A, Hussain R M, Ur R N, et al. De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.). DNA Research (6):6.
For Research Use Only. Not for use in diagnostic procedures.

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