Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Pyruvic Acid Analysis Service

Pyruvic Acid Analysis Service

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Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

About Pyruvic Acid

Pyruvate (CH3COCOOH) is the simplest α-keto acid, with carboxylic acid and ketone functional groups.

Pyruvate can be produced from glucose by glycolysis, can be converted back to carbohydrates (such as glucose) by gluconeogenesis, or can be converted to fatty acids by reaction with acetyl-CoA. It can also be used to construct the amino acid alanine, which can be converted into ethanol or lactic acid by fermentation.

When oxygen is present (aerobic respiration), pyruvate provides energy to cells through the citric acid cycle (also known as the Krebs cycle), or ferments to produce lactic acid (lactic acid) in the absence of oxygen.

Its chemical formula is C3H4O3, and CAS number is 127-17-3.

MS-based technical means can realize the qualitative and quantitative analysis of pyruvic acid.

Molecular structure of pyruvic acidMolecular structure of pyruvic acid

Technical Route of Targeted Metabolomics of Pyruvic Acid

Technical Route of Targeted Metabolomics of Pyruvic Acid

Sample Requirements

Sample type
Plants, yeasts, microorganisms, etc.
     Serum, plasma, urine, bile, bile acid, etc.
     Cells, liver, brain tissue and other animal tissues and feces, etc.
     There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.
Sample demand
Plant  root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried  sample > 0.5 g
            Cells  / microorganisms / cell supernatant / culture medium supernatant: the number  of cells or bacteria > 107, supernatant > 2 ml
            Serum  / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and  other homogeneous liquid samples > 200 µl.
            (Hemolysis  should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
            Animal  tissue / feces related samples > 200mg / sample
For other sample types, please consult technical support or sales.
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
    ※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

  • Experimental steps
  • Related mass spectrometry parameters
  • Part of the mass spectrum picture
  • Raw data

Project Cycle

  • A standard experiment and analysis process takes about 1~4 weeks.

Creative Proteomics offers several approaches to metabolomics studies, delivers precise and detailed data and analysis report. We can also customize the methods or establish new methods together with our collaborators, so they are fit-for-purpose and meet your specific needs. If you have any questions or specific requirements, please feel free to contact us.

References

  1. Hatoum D, Annaratone C, Hertog M, et al. Targeted metabolomics study of ‘Braeburn’apples during long-term storage. Postharvest Biology and Technology, 2014, 96: 33-41.
  2. Griffiths W J, Koal T, Wang Y, et al. Targeted metabolomics for biomarker discovery. Angewandte Chemie International Edition, 2010, 49(32): 5426-5445.
  3. Mohammed A, Hussain R M, Ur R N, et al. De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.). DNA Research (6):6.
For Research Use Only. Not for use in diagnostic procedures.

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