Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Malic Acid Analysis Service

Malic Acid Analysis Service

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Creative Proteomics' targeted metabolomics services target specific metabolites and pathways of interest, which can accurately monitor dynamic metabolic processes, reveal related metabolic mechanisms and verify potential metabolic biomarkers, to guide your research with reliable and accurate measurement results.

About Malic Acid

Malic acid, also known as 2-hydroxysuccinic acid, has strong hygroscopicity and is easily soluble in water and ethanol. It is mainly used in the food industry and the pharmaceutical industry.

Since there is an asymmetric carbon atom in the malic acid molecule, there are two stereoisomers. In nature, it exists in three forms, namely D-malic acid, L-malic acid and DL-malic acid.

Naturally occurring malic acid is L-shaped, and it is present in almost all fruits, with the most in pome fruit.

Its chemical formula is C4H6O5, and CAS number is 6915-15-7.

MS-based technical means can realize the qualitative and quantitative analysis of malic acid.

Molecular structure of malic acidMolecular structure of malic acid

Technical Route of Targeted Metabolomics of Malic Acid

Technical Route of Targeted Metabolomics of Malic Acid

Sample Requirements

Sample type
Plants, yeasts, microorganisms, etc.
     Serum, plasma, urine, bile, bile acid, etc.
     Cells, liver, brain tissue and other animal tissues and feces, etc.
     There is no restriction on plant varieties. For varieties with special requirements or rare varieties, please contact our staff for more information.
Sample demand
Plant root / stem / leaf / fruit / seed > 200 mg fresh weight, freeze-dried sample > 0.5 g
Cells / microorganisms / cell supernatant / culture medium supernatant: the number of cells or bacteria > 107, supernatant > 2 ml
Serum / plasma / urine / ruminal fluid / cerebrospinal fluid / amniotic fluid and other homogeneous liquid samples > 200 µl.
(Hemolysis should be avoided. Cerebrospinal fluid can be as low as 100 µl.)
Animal tissue / feces related samples > 200mg / sample
For other sample types, please consult technical support or sales.
Sample mixed and repeat
In order to ensure the accuracy of the samples and reduce systematic errors during sampling, it is necessary to select more than 3 materials with the same condition for each sample.
    ※ The same condition refers to the same period, basically the same phenotype, and the same part.
It is recommended that prepare more than 6 biological replicates.
Storage and transportation
Quick-frozen preservation with liquid nitrogen can minimize the leakage time of plant samples at room temperature, and store it at -80℃.
Dry ice transportation (about 3~4 kg dry ice is consumed every day, please use sufficient dry ice for transportation).

Feedback to Customers

  • Experimental steps
  • Related mass spectrometry parameters
  • Part of the mass spectrum picture
  • Raw data

Project Cycle

  • A standard experiment and analysis process takes about 1~4 weeks.

References

  1. Pawlak M, Klupczynska A, Kokot Z J, et al. Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids. Toxins, 2019, 12(1):14.
  2. Xin, Wang, Tao, et al. Targeted Metabolomic Profiling Reveals Association Between Altered Amino Acids and Poor Functional Recovery After Stroke. Frontiers in neurology, 2019, 10:1425-1425.
  3. Wang X, Zhao X, Zhao J, et al. Serum metabolite signatures of epithelial ovarian cancer based on targeted metabolomics. Clinica Chimica Acta, 2021, 518: 59-69.
For Research Use Only. Not for use in diagnostic procedures.

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