Phytic Acid Analysis

What is Phytic Acid?

Phytic acid is a phosphorus-rich compound that serves as the primary storage form of phosphorus in plants. Its intricate molecular structure consists of six phosphate groups surrounding an inositol ring, contributing to its ability to bind to minerals such as calcium, zinc, and iron. While its anti-nutrient properties can impede mineral absorption, phytic acid has also been linked to potential health benefits, including antioxidant and anticancer properties.

Analyzing phytic acid content and metabolism holds immense importance in various fields, ranging from nutrition and food science to pharmaceuticals and agriculture. By gaining insights into its presence and behavior, researchers and industries can make informed decisions to optimize nutrient bioavailability and develop innovative products.

Understanding the metabolic fate of phytic acid is crucial for unraveling its effects on health and nutrition. Creative Proteomics offers a range of specialized services to analyze phytic acid metabolism, shedding light on its breakdown products, metabolic pathways, and potential interactions within biological systems. Through advanced techniques and state-of-the-art instrumentation, our experts provide in-depth insights into the dynamic processes involving phytic acid.

Phytic Acid Analysis in Creative Proteomics

1. Metabolite Profiling

Our metabolite profiling services provide a panoramic view of phytic acid and its derivatives within complex biological matrices. Leveraging advanced mass spectrometry and chromatography techniques, we offer a detailed snapshot of the metabolic landscape, identifying key metabolites and their concentrations.

2. Metabolic Pathway Analysis

Navigating the labyrinthine pathways of phytic acid metabolism requires more than just data – it demands insights. Our skilled bioinformaticians and biologists collaborate to dissect the metabolic pathways, unraveling the sequential enzymatic reactions that guide phytic acid's transformation. This analysis not only enhances our understanding of phytic acid but also sheds light on its potential interactions within biological systems.

3. Quantitative Analysis

Precision is paramount in unraveling the complexities of phytic acid metabolism. Our quantitative analysis services utilize high-resolution mass spectrometry to quantify phytic acid and its metabolites accurately. This quantitative data forms the bedrock for robust research and informed decision-making.

4. Isotope Labeling Studies

Dive deeper into the metabolic fate of phytic acid with our isotope labeling studies. By introducing stable isotopes, we trace the fate of phytic acid and gain insights into its transformational journey, providing a dynamic perspective on its interactions and potential applications.

5. Comparative Metabolomics

Context is key in metabolomics, and our comparative metabolomics services provide just that. By comparing phytic acid metabolism across different samples or conditions, we unearth valuable information about the compound's adaptability and potential functional implications.

6. Biomarker Discovery

Discover the untapped potential of phytic acid as a biomarker with our biomarker discovery services. Through rigorous analysis of metabolic signatures, we identify unique markers associated with phytic acid metabolism, contributing to advancements in diagnostics and personalized medicine.

Phytic Acid Assay Platforms

Liquid Chromatography-Mass Spectrometry (LC-MS)

Instrument: Agilent 1290 Infinity II LC System coupled with the Q-Exactive HF-X mass spectrometer.

Advantages:

• Separation Efficiency: LC separates complex mixtures, enabling the analysis of various phytic acid derivatives present in samples.
• High Sensitivity: Q-Exactive HF-X's high-resolution MS detects low-concentration metabolites, providing a comprehensive view of phytic acid's metabolomic landscape. (Detection Limit: as low as 1 ng/mL)
• Quantitative Accuracy: LC-MS facilitates precise quantification of phytic acid and its derivatives, crucial for nutritional and metabolic studies.

Gas Chromatography-Mass Spectrometry (GC-MS)

Instrument: Agilent 7890B Gas Chromatograph coupled with the Agilent 5977A Mass Selective Detector.

Advantages:

• Volatile Compound Analysis: GC-MS excels in volatile compound analysis, making it suitable for volatile phytic acid derivatives.
• High Specificity: Mass selective detector provides specific and accurate identification of phytic acid metabolites.
• Robustness: GC-MS is robust and reproducible, ensuring consistent results even in complex matrices. (Detection Limit: in the low picogram range)

Triple Quadrupole Mass Spectrometry (QQQ-MS)

Instrument: Agilent 6495 Triple Quadrupole LC/MS System.

Advantages:

• Targeted Analysis: QQQ-MS enables precise quantification and confirmation of specific phytic acid metabolites.
• Enhanced Sensitivity: Triple quadrupole configuration enhances sensitivity for trace-level quantification. (Detection Limit: as low as 0.1 ng/mL)
• Quantitative Precision: Offers exceptional accuracy and precision in quantifying target analytes.

Time-of-Flight Mass Spectrometry (TOF-MS)

Instrument: Waters Xevo G2-XS QToF Mass Spectrometer.

Advantages:

• High-Resolution Data: TOF-MS provides high-resolution mass data, enabling accurate determination of phytic acid and its various forms. (Mass Accuracy: within 2 ppm)
• Wide Mass Range: Capable of detecting a broad range of metabolites, allowing comprehensive analysis of phytic acid metabolism.
• Metabolite Identification: High-resolution TOF-MS aids in the identification of unknown or unexpected metabolites.

Sample Requirements for Phytic Acid Assay

If you would like to analyze other organic acids or learn more, please contact us. We look forward to working with you.