Gardenia jasminoides contains more than 40 kinds of bioactive substances, mainly iridoid glycosides and volatile oil, among which the active components are iridoid glycosides. Gardenoside is the highest content of iridoid glycosides in gardenia jasminoides.
Gardenoside has a wide range of pharmacological effects and has great development value. Gardenoside has analgesic and anti-inflammatory effects. It has a significant neutralizing effect on LPS, can significantly inhibit the release of LPS induced inflammatory factors, and antagonize the damage effect of LPS on the body. Also can reduce hepatic free radical generation and enhance free radical scavenging ability, inhibit inflammatory factor release, promote bile secretion and excretion, and prevent hepatocyte damage. In addition, it also has antioxidant, anti-diabetic and Alzheimer's disease treatment effects.
At present, the research on gardenoside only stays in the primary stage. In order to develop new drugs, it is necessary to systematically study the pharmacological effects of gardenoside. Furthermore, an indication with definite curative effect and obvious advantages compared with existing clinical drugs will be screened out for further study, so as to determine the development direction of gardenoside as soon as possible.
Gardenosides mainly play pharmacological effect through its metabolite kynepin, and kynepin is also the main causative factor of liver toxicity. How to avoid or alleviate the toxicity on the basis of guaranteeing the pharmacological activity of gardenoside is also the urgent problem that needs to be solved to develop the medicinal value of gardenoside.
To accelerate your project, Creative Proteomics can provide you with qualitative and quantitative analysis of gardenoside based on the utilization of the UHPLC-Q-Exactive MS technology platform. We have chosen high resolution extraction ion chromatography (HREICs) and parallel reaction monitoring mode (PRM) to acquire data for comparison with gardenoside standards for the identification of gardenoside metabolites.
Quantification of gardenoside by isotope internal standard based on UHPLC-Q-Exactive MS platform
- Serum / plasma: ≥ 100 ul/sample
- Animal tissue: ≥ 50 mg/sample
- Urine: ≥ 100 ul/sample
- Feces: ≥ 50 mg/sample
- Saliva: ≥ 100 ul/sample
- Cell: ≥ 1×107
- Plant samples: ≥ 200 mg
We can also provide other sample types for analysis. Please contact our technicians for specific sample types and content requirements.
- Experimental procedure
- Parameters of liquid chromatography and mass spectrometry
- Chromatography and mass spectrometry raw data
- Chromatogram and mass spectrum
- Gardenoside quantification data
- Custom analysis report
Turnaround Time: 1-4 weeks
Creative Proteomics offers several approaches to provide gardenoside analysis service and deliver precise and detailed data and analysis report. If you want to detect other compounds, you can tell us through the inquiry form, and our technicians will communicate with you.
For Research Use Only. Not for use in diagnostic procedures.