Geniposide Analysis Service

The iridoids geniposide and genipin, the secondary metabolites of monoterpenoids, have various pharmacological activities and wide application value.

Geniposide and Genipin

In general, the higher levels of glycosides are not components that act directly in the blood, but their metabolites, aglycones and their derivatives. After the drug is taken, it changes into another structure under the action of digestive system enzymes, stomach acid, intestinal bacteria, intestinal membrane enzymes, etc., and enters the blood to take effect. For example, the geniposide in gardenia must be transformed into kynepin by β glucosidase of intestinal bacteria in order to be absorbed and thus play the medicinal effect of promoting bile secretion. Accurate detection of geniposide metabolites in urine, blood and other samples will facilitate the progress of related research.

Genipin is a natural crosslinking agent extracted from plants, with multiple active functional groups and low cytotoxicity. The ring crosslinks formed by Genipin are more stable than other reticular and linear crosslinks.

Geniposide have a wide range of pharmacological activities such as hypoglycemic and lipid-regulating, neurotrophic and neuroprotective, hepatoprotective, anti-Alzheimer's, and anticancer. Studies with geniposide and genipin as precursors, through a series of chemical reactions, can lead to compounds with similar chemical backbones, providing a shortcut to find better therapeutic drugs.

In order to facilitate further research and exploitation of kynepin and provide scientific basis for clinical applications, Creative Proteomics has established a dedicated analytical platform to achieve absolute quantitative analysis of geniposide and geniposide metabolites. We obtain accurate data results by using a high-resolution UHPLC-Q-Exactive MS instrument in parallel reaction monitoring (PRM) mode in combination with geniposide standards.

Service Workflow

Quantification of geniposide by isotope internal standard based on UHPLC-Q-Exactive MS platform

Sample Requirements

• Serum / plasma: ≥ 100 ul/sample
• Animal tissue: ≥ 50 mg/sample
• Urine: ≥ 100 ul/sample
• Feces: ≥ 50 mg/sample
• Saliva: ≥ 100 ul/sample
• Cell: ≥ 1×10⁷
• Plant samples: ≥ 200 mg

We can also provide other sample types for analysis. Please contact our technicians for specific sample types and content requirements.

Deliverables

• Experimental procedure
• Parameters of liquid chromatography and mass spectrometry
• Chromatography and mass spectrometry raw data
• Chromatogram and mass spectrum
• Geniposide quantification data
• Custom analysis report

Turnaround Time: 1-4 weeks

Creative Proteomics offers several approaches to provide geniposide analysis service and deliver precise and detailed data and analysis report. If you want to detect other compounds, you can tell us through the inquiry form, and our technicians will communicate with you.