Metabolomics Creative Proteomics

Creative Proteomics Metabolomics

Chlorophyll c2 Analysis Service

Chlorophyll c2 Analysis Service

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About chlorophyll c2

Chlorophyll c2 is an organic compound with the molecular formula C35H28MgN4O5, and CAS number is 27736-03-4.

Chlorophyll c2 is mainly distributed in brown algae, brown flagella, yellow flagella, diatoms and yellow-green algae.

Chlorophyll c2 participates in photosystem II as an auxiliary pigment in diatoms and brown algae.

Targeted metabolomics is mainly used to identify and quantitatively analyze specific metabolites in samples, this method has been widely used to analyze and compare multiple target metabolites in different physiological states. It is a key analysis method for researches on green plants to improve nutrition and so on. Based on the LC-MS/MS platform, Creative Proteomics use analytical standard detection methods to perform accurate qualitative and quantitative analysis on the pre-screened and validated or identified potential biomarkers (chlorophyll c2).

Molecular structure of chlorophyll c2 Molecular structure of chlorophyll c2

Sample requirements

Algae samples need to prepare 30-40mg freeze-dried powder or 300mg fresh samples.

Plant tissues need to be mixed from more than 3 plants as a sample. Plant samples need to be freeze-dried, please try to provide more than 3g (fresh weight).

The samples should be stored at -80°, transported on dry ice, and cannot be frozen and thawed repeatedly.

Biological duplication

If it is a plant or microorganism sample, no less than 6 biological replicates.

If it is a Algae sample, no less than 6 biological replicates.

Workflow of targeted metabolomics of chlorophyll c2

Molecular structure of chlorophyll c2 Workflow of targeted metabolomics of chlorophyll c2

Design experiments and sample processing

Standard preparation and mass spectrometry detection

Make standard curve

Extraction of metabolites in samples

Raw data preprocessing

Qualitative and quantitative target metabolites

Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)

Extended analysis, such as metabolite screening, KEGG pathway analysis and hierarchical clustering

Provided by Creative Proteomics

Detailed technical report, including instrument model and parameters, reagents used, experimental procedures, data analysis results, etc.

Raw data of target metabolism sequencing.

Project cycle

About 1-3 weeks

Technical advantages

Qualitatively accurate

   Check the secondary spectra of sample materials and of standard products one by one.

Quantitatively accurate

   Choose the best ion pair to ensure accurate quantification of the substance.

High sensitivity

   Based on advanced mass spectrometry platform to ensure high sensitivity.

Targeted metabolomics based on mass spectrometry can more sensitively and more accurately quantify target metabolites. With decades of experience in mass spectrometry services, Creative Proteomics undertakes the qualitative and quantitative analysis of a variety of plant metabolites, provides accurate and detailed data and analysis reports. From sample extraction and detection to bioinformatics, Creative Proteomics can meet your special needs, and has a good record. If you want to know more, please contact us immediately.

References

  1. Liu B, Xiu un Wang, Cao Y, et al. Factors affecting freezing tolerance: a comparative transcriptomics study between field and artificial cold acclimations in overwintering evergreens[J]. The Plant Journal, 2020.
  2. Yu C, Zhao X, Qi G, et al. Integrated analysis of transcriptome and metabolites reveals an essential role of metabolic flux in starch accumulation under nitrogen starvation in duckweed[J]. Biotechnology for Biofuels, 2017, 10(1).
  3. Jin D, Dai K, Xie Z, et al. Secondary Metabolites Profiled in Cannabis Inflorescences, Leaves, Stem Barks, and Roots for Medicinal Purposes[J]. Scientific Reports, 2020, 10(1).
For Research Use Only. Not for use in diagnostic procedures.

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