About chlorophyll c2
Chlorophyll c2 is an organic compound with the molecular formula C35H28MgN4O5, and CAS number is 27736-03-4.
Chlorophyll c2 is mainly distributed in brown algae, brown flagella, yellow flagella, diatoms and yellow-green algae.
Chlorophyll c2 participates in photosystem II as an auxiliary pigment in diatoms and brown algae.
Targeted metabolomics is mainly used to identify and quantitatively analyze specific metabolites in samples, this method has been widely used to analyze and compare multiple target metabolites in different physiological states. It is a key analysis method for researches on green plants to improve nutrition and so on. Based on the LC-MS/MS platform, Creative Proteomics use analytical standard detection methods to perform accurate qualitative and quantitative analysis on the pre-screened and validated or identified potential biomarkers (chlorophyll c2).
Molecular structure of chlorophyll c2
Sample requirements
Algae samples need to prepare 30-40mg freeze-dried powder or 300mg fresh samples.
Plant tissues need to be mixed from more than 3 plants as a sample. Plant samples need to be freeze-dried, please try to provide more than 3g (fresh weight).
The samples should be stored at -80°, transported on dry ice, and cannot be frozen and thawed repeatedly.
Biological duplication
If it is a plant or microorganism sample, no less than 6 biological replicates.
If it is a Algae sample, no less than 6 biological replicates.
Workflow of targeted metabolomics of chlorophyll c2
Workflow of targeted metabolomics of chlorophyll c2
Design experiments and sample processing
Standard preparation and mass spectrometry detection
Make standard curve
Extraction of metabolites in samples
Raw data preprocessing
Qualitative and quantitative target metabolites
Bioinformatics analysis (PCA analysis, cluster analysis, difference analysis, etc.)
Extended analysis, such as metabolite screening, KEGG pathway analysis and hierarchical clustering
Provided by Creative Proteomics
Detailed technical report, including instrument model and parameters, reagents used, experimental procedures, data analysis results, etc.
Raw data of target metabolism sequencing.
Project cycle
About 1-3 weeks
Technical advantages
Qualitatively accurate
Check the secondary spectra of sample materials and of standard products one by one.
Quantitatively accurate
Choose the best ion pair to ensure accurate quantification of the substance.
High sensitivity
Based on advanced mass spectrometry platform to ensure high sensitivity.
Targeted metabolomics based on mass spectrometry can more sensitively and more accurately quantify target metabolites. With decades of experience in mass spectrometry services, Creative Proteomics undertakes the qualitative and quantitative analysis of a variety of plant metabolites, provides accurate and detailed data and analysis reports. From sample extraction and detection to bioinformatics, Creative Proteomics can meet your special needs, and has a good record. If you want to know more, please contact us immediately.
For Research Use Only. Not for use in diagnostic procedures.
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